PrimePCR™ SYBR® Green Assay: SNX3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040495

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SNX3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SNX3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037772
Assay Design:   exonic
Chromosome Location:   6:108533202-108533321question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000230085 ENST00000426155 ENST00000349379

Gene Information

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain which is a phosphoinositide binding domain and are involved in intracellular trafficking. This protein does not contain a coiled coil region like most family members. This protein interacts with phosphatidylinositol-3-phosphate and is involved in protein trafficking. [provided by RefSeq Jul 2008]

Gene Symbol:   SNX3
Gene Name:   sorting nexin 3
Aliases:   Grd19, MCOPS8, MGC17570, SDP3
RefSeq:   NC_000006.11 NG_023278.1 NT_025741.15
Ensembl:   ENSG00000112335
Entrez:   8724
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.420000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download