PrimePCR™ SYBR® Green Assay: EIF3CL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040103

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EIF3CL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EIF3CL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037380
Assay Design:   exonic
Chromosome Location:   16:28394584-28394731question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000371500 ENST00000380876 ENST00000398944 ENST00000398943 ENST00000392945 ENST00000354753 ENST00000440944 ENST00000421085 ENST00000559074 ENST00000379122 ENST00000507224 ENST00000504154 ENST00000404767 ENST00000389470 ENST00000413379 ENST00000598840 ENST00000270225 ENST00000392776 ENST00000360772 ENST00000346322 ENST00000475616 ENST00000360304 ENST00000359169 ENST00000354792 ENST00000323851 ENST00000414097 ENST00000354944 ENST00000395587 ENST00000566866 ENST00000564243 ENST00000331666 ENST

Gene Information

Description Not Available

Gene Symbol:   EIF3CL
Gene Name:   eukaryotic translation initiation factor 3, subunit C-like
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000205609
Entrez:   728689
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.810000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download