PrimePCR™ SYBR® Green Assay: LY6G6C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040040

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037317
Assay Design:   exonic
Chromosome Location:   6:31686680-31686795question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000542440 ENST00000267197 ENST00000374401 ENST00000259006 ENST00000578402 ENST00000583211 ENST00000578061 ENST00000378609 ENST00000530885 ENST00000227451 ENST00000448386 ENST00000337435 ENST00000437912 ENST00000470881 ENST00000419939 ENST00000415229 ENST00000301776 ENST00000495859 ENST00000375819 ENST00000480025 ENST00000428121 ENST00000467714 ENST00000428498 ENST00000360948 ENST00000357724 ENST00000497123 ENST00000434915 ENST00000513429 ENST00000371130 ENST00000422452 ENST00000383413 ENST

Gene Information

LY6G6C belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al. 2002 [PubMed 12079290]).[supplied by OMIM Mar 2008]

Gene Symbol:   LY6G6C
Gene Name:   lymphocyte antigen 6 complex, locus G6C
Aliases:   C6orf24, G6c, NG24
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204421
Entrez:   80740
UniGene:   Hs.732272
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.310000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download