PrimePCR™ SYBR® Green Assay: AGAP4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039959

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037236
Assay Design:   exonic
Chromosome Location:   10:46342573-46342663question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000456984 ENST00000453919 ENST00000577090 ENST00000602930 ENST00000425119 ENST00000479304 ENST00000448048 ENST00000492347 ENST00000374095 ENST00000412531 ENST00000374056 ENST00000452145 ENST00000413193 ENST00000355232 ENST00000374232 ENST00000440460 ENST00000443782 ENST00000374094 ENST00000472090 ENST00000426433 ENST00000562557 ENST00000342551 ENST00000579115 ENST00000311652 ENST00000584334 ENST00000581330

Gene Information

Description Not Available

Gene Symbol:   AGAP4
Gene Name:   ArfGAP with GTPase domain, ankyrin repeat and PH domain 4
Aliases:   CTGLF1, MRIP2
RefSeq:   NC_000010.10 NT_033985.7
Ensembl:   ENSG00000188234
Entrez:   119016
UniGene:   Hs.656384
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download