PrimePCR™ SYBR® Green Assay: SLC1A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039065

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036341
Assay Design:   exonic
Chromosome Location:   5:36608431-36608525question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000265113 ENST00000513903 ENST00000416645 ENST00000505202 ENST00000513646 ENST00000381918

Gene Information

This gene encodes a member of a member of a high affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq Nov 2009]

Gene Symbol:   SLC1A3
Gene Name:   solute carrier family 1 (glial high affinity glutamate transporter), member 3
Aliases:   EA6, EAAT1, FLJ25094, GLAST, GLAST1
RefSeq:   NC_000005.9 NT_006576.16 NG_015890.1
Ensembl:   ENSG00000079215
Entrez:   6507
UniGene:   Hs.481918
Chromosome Mapping:   5p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.710000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download