PrimePCR™ SYBR® Green Assay: ZCCHC13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0034323
Assay Design:   Exonic
Chromosome Location:   X:73524395-73524604question
Amplicon Length:   180

Gene Information

This gene appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 3. However the CDS of this intronless gene remains relatively intact it is conserved in other mammalian species it is known to be transcribed and it is therefore thought to encode a functional protein. The encoded protein contains six CCHC-type zinc fingers and is thus thought to function as a transcription factor. [provided by RefSeq May 2010]

Gene Symbol:   ZCCHC13
Gene Name:   zinc finger, CCHC domain containing 13
Aliases:   CNBP2, ZNF9L
RefSeq:   NC_000023.10 NG_021228.1 NT_011669.17
Ensembl:   ENSG00000187969
Entrez:   389874
UniGene:   Hs.157231
Chromosome Mapping:   Xq13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download