This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: FOS, Human
PrimePCR™ Template for SYBR® Green Assay: FOS, Human
The Fos gene family consists of 4 members: FOS FOSB FOSL1 and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family thereby forming the transcription factor complex AP-1. As such the FOS proteins have been implicated as regulators of cell proliferation differentiation and transformation. In some cases expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.