PrimePCR™ SYBR® Green Assay: FOS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0023033
Assay Design:   Exonic
Chromosome Location:   14:75747997-75748124question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000303562 ENST00000535987 ENST00000555686 ENST00000555347

Gene Information

The Fos gene family consists of 4 members: FOS FOSB FOSL1 and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family thereby forming the transcription factor complex AP-1. As such the FOS proteins have been implicated as regulators of cell proliferation differentiation and transformation. In some cases expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq Jul 2008]

Gene Symbol:   FOS
Gene Name:   FBJ murine osteosarcoma viral oncogene homolog
Aliases:   AP-1, C-FOS
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000170345
Entrez:   2353
UniGene:   Hs.719809
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download