This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: RASGRP2, Human
PrimePCR™ Template for SYBR® Green Assay: RASGRP2, Human
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain 2 tandem repeats of EF-hand calcium-binding motifs and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.