PrimePCR™ SYBR® Green Assay: ITSN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004522
Assay Design:   Exonic
Chromosome Location:   21:35138179-35138291question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000399353 ENST00000381318 ENST00000381291 ENST00000399367 ENST00000399352 ENST00000399355 ENST00000399349 ENST00000381283 ENST00000381285 ENST00000381288 ENST00000437442 ENST00000399326 ENST00000381289 ENST00000379960 ENST00000399338

Gene Information

The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin CDC42 SNAP23 SNAP25 SPIN90 EPS15 EPN1 EPN2 and STN2. Multiple transcript variants encoding different isoforms have been found for this gene but the full-length nature of only two of them have been characterized so far. [provided by RefSeq Jul 2008]

Gene Symbol:   ITSN1
Gene Name:   intersectin 1 (SH3 domain protein)
Aliases:   ITSN, MGC134948, MGC134949, SH3D1A, SH3P17
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000205726
Entrez:   6453
UniGene:   Hs.160324
Chromosome Mapping:   21q22.1-q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download