PrimePCR™ SYBR® Green Assay: ASIP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024697

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ASIP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ASIP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003422
Assay Design:   Exonic
Chromosome Location:   20:32848220-32850641question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000374954

Gene Information

In mice the agouti gene encodes a paracrine signaling molecule that causes hair follicle melanocytes to synthesize pheomelanin a yellow pigment instead of the black or brown pigment eumelanin. Pleiotropic effects of constitutive expression of the mouse gene include adult-onset obesity increased tumor susceptibility and premature infertility. This gene is highly similar to the mouse gene and encodes a secreted protein that may (1) affect the quality of hair pigmentation (2) act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (3) play a role in neuroendocrine aspects of melanocortin action and (4) have a functional role in regulating lipid metabolism in adipocytes. [provided by RefSeq Jul 2008]

Gene Symbol:   ASIP
Gene Name:   agouti signaling protein
Aliases:   AGSW, AGTI, AGTIL, ASP, MGC126092, MGC126093, SHEP9
RefSeq:   NC_000020.10 NG_011439.1 NT_011362.10
Ensembl:   ENSG00000101440
Entrez:   434
Chromosome Mapping:   20q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download