This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: MED19, Human
PrimePCR™ Template for SYBR® Green Assay: MED19, Human
MED19 is a component of the Mediator complex which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al. 2003 [PubMed 12584197]).[supplied by OMIM Oct 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.