This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0025834
PrimePCR™ PreAmp for SYBR® Green Assay: ACTR1B, Human
PrimePCR™ Template for SYBR® Green Assay: ACTR1B, Human
This gene encodes a 42.3 kD subunit of dynactin a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions including ER-to-Golgi transport the centripetal movement of lysosomes and endosomes spindle formation chromosome movement nuclear positioning and axonogenesis. This subunit like ACTR1A is an actin-related protein. These two proteins which are of equal length and share 90% amino acid identity are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq Aug 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.