PrimePCR™ SYBR® Green Assay: LMO3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002545
Assay Design:   Exonic
Chromosome Location:   12:16753645-16753736question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000441439 ENST00000447609 ENST00000320122 ENST00000540848 ENST00000535535 ENST00000537304 ENST00000534946 ENST00000540445 ENST00000541846 ENST00000539534 ENST00000546281 ENST00000537757 ENST00000546279 ENST00000538051 ENST00000545436 ENST00000540590 ENST00000538020 ENST00000541295 ENST00000261169 ENST00000354662 ENST00000542544

Gene Information

The protein encoded by this gene belongs to the rhombotin family of cysteine-rich LIM domain oncogenes. This gene is predominantly expressed in the brain. Related family members LMO1 and LMO2 on chromosome 11 have been reported to be involved in chromosomal translocations in T-cell leukemia. Many alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   LMO3
Gene Name:   LIM domain only 3 (rhombotin-like 2)
Aliases:   DAT1, MGC26081, RBTN3, RBTNL2, RHOM3, Rhom-3
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000048540
Entrez:   55885
UniGene:   Hs.504908
Chromosome Mapping:   12p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.590000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download