PrimePCR™ SYBR® Green Assay: DDX20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: DDX20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DDX20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001119
Assay Design:   Exonic
Chromosome Location:   1:112305307-112305532question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000369702 ENST00000475700 ENST00000536167

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN the spinal muscular atrophy gene product and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq Jul 2008]

Gene Symbol:   DDX20
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Aliases:   DKFZp434H052, DP103, GEMIN3
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000064703
Entrez:   11218
Chromosome Mapping:   1p21.1-p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download