PrimePCR™ SYBR® Green Assay: CER1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CER1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CER1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0050198
Assay Design:   Exonic
Chromosome Location:   1:422056-422245question
Amplicon Length:   160
Splice Variants Targeted:   AT2G02570.1 AT2G02570.2 AT2G02570.3 AT2G02570.4 AT1G02205.1 AT1G02205.2 AT1G02205.3

Gene Information

Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.

Gene Symbol:   CER1
Gene Name:   Fatty acid hydroxylase superfamily
Aliases:   Not Available
RefSeq:   NM_001197961 NM_100101 NM_180601
Ensembl:   AT1G02205
Entrez:   837602
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download