PrimePCR™ SYBR® Green Assay: NRT1.1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: NRT1.1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NRT1.1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0040256
Assay Design:   Exonic
Chromosome Location:   1:4106141-4106251question
Amplicon Length:   81
Splice Variants Targeted:   AT1G12110.1

Gene Information

Encodes NRT1.1 (CHL1), a dual-affinity nitrate transporter. The protein is expressed in guard cells and function in stomatal opening. Mutants have less transpiration and are more tolerant to drought. Expressed in lateral roots. Involved in nitrate signaling which enables the plant root system to detect and exploit nitrate-rich soil patches. Comparing to the wild type, the mutant displays a strongly decreased lateral root proliferation phenotype in nitrate rich patches on growth medium.

Gene Symbol:   NRT1.1
Gene Name:   Nitrate transporter 1.1
Aliases:   Not Available
RefSeq:   NM_101083
Ensembl:   AT1G12110
Entrez:   837763
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download