PrimePCR™ SYBR® Green Assay: MMZ1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: MMZ1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MMZ1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0037112
Assay Design:   Exonic
Chromosome Location:   1:8258571-8258678question
Amplicon Length:   78
Splice Variants Targeted:   AT1G23260.1

Gene Information

MMZ1/UEV1A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UEV1A can form diubiquitin and triubiquitin chains in combination with UBC13A/UBC35 in vitro. It can also functionally complement an mms2 mutation in budding yeast, both by increasing mms2 mutant viability in the presence of the DNA damaging agent MMS, and by reducing the rate of spontaneous DNA mutation. However, a combination of MMZ1/UEV1A and UBC13A do not do a good job of rescuing an mms2 ubc13 double mutant in yeast. MMZ1/UEV1A transcripts are found at low levels in most plant organs, but cannot be detected in the pollen. Transcript levels do not appear to be stress-inducible. The uev1a-1 mutant shows normal sensitivity to MMS in germination assays suggesting that UEV1A is not required for DNA damage tolerance during this developmental stage.

Gene Symbol:   MMZ1
Gene Name:   MMS ZWEI homologue 1
Aliases:   Not Available
RefSeq:   NM_102175
Ensembl:   AT1G23260
Entrez:   838935
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download