PrimePCR™ SYBR® Green Assay: HCF173, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: HCF173, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HCF173, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0034007
Assay Design:   Exonic
Chromosome Location:   1:5726092-5726315question
Amplicon Length:   116
Splice Variants Targeted:   AT1G16720.1

Gene Information

Encodes HCF173, a protein with weak similarities to the superfamily of the short-chain dehydrogenases/reductases. HCF173 is involved in the initiation of translation of the psbA mRNA. Mutants shows a high chlorophyll fluorescence phenotype (hcf) and are severely affected in the accumulation of PSII subunits. The protein HCF173 is localized in the chloroplast, where it is mainly associated with the membrane system and is part of a higher molecular weight complex with psbA mRNA as a component of this complex.

Gene Symbol:   HCF173
Gene Name:   High chlorophyll fluorescence phenotype 173
Aliases:   Not Available
RefSeq:   NM_101533
Ensembl:   AT1G16720
Entrez:   838243
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download