PrimePCR™ SYBR® Green Assay: DDF1, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0031928
Assay Design:   Exonic
Chromosome Location:   1:4290199-4290312question
Amplicon Length:   84
Splice Variants Targeted:   AT1G12610.1

Gene Information

Encodes a member of the DREB subfamily A-1 of ERF/AP2 transcription factor family (DDF1). The protein contains one AP2 domain. There are six members in this subfamily, including CBF1, CBF2, and CBF3. Overexpression of this gene results in delayed flowering and dwarfism, reduction of gibberellic acid biosynthesis, and increased tolerance to high levels of salt. This gene is expressed in all tissues examined, but most abundantly expressed in upper stems. Overexpression of this gene is also correlated with increased expression of GA biosynthetic genes and RD29A (a cold and drought responsive gene). Under salt stress it induces the expression of GAOX7, which encodes ad C20-GA inhibitor.

Gene Symbol:   DDF1
Gene Name:   Integrase-type DNA-binding superfamily protein
Aliases:   Not Available
RefSeq:   NM_101131
Ensembl:   AT1G12610
Entrez:   837817
UniGene:   At.42068
Chromosome Mapping:   Not Available

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