Bio-Rad Cell Lysate Controls* Selection Guide

Phosphoprotein Lysate Control Catalog #
Akt (Ser473)
Akt (Thr308)
Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)
GSK-3α/β (Ser21/Ser9)
MEK1 (Ser217/Ser221)
EGF-treated HEK-293 171-YZ0001
ATF-2 (Tyr71)
c-Jun (Ser63)
CREB (Ser133)
JNK (Thr183/Tyr185)
p38 MAPK (Thr180/Tyr182)
p53 (Ser15)
UV-treated HEK-293 171-YZ0009
BAD (Ser136)
IRS-1 (Ser636/Ser639)
mTOR (Ser2448)
PDGFR-α (Tyr754)
PDGFR-β (Tyr751)
PTEN (Ser380)
PDGF-treated NIH3T3 171-YZ0007
Btk (Tyr223)
Lyn (Tyr507)
Pl3K p85 (Tyr458)
Syk (Tyr352)
H2O2-treated Ramos 171-YZ0011
c-AbI (Tyr245) Untreated K-562 171-YZT003
EGFR (Tyr1068)
EGFR (Tyr1173)
EGF-treated HeLa 171-YZ0002
HER-2 (Tyr1248)
HSP27 (Ser78)
p90 RSK (Ser380)
S6 ribosomal protein (Ser235/Ser236)
EGF-treated SK-Br3 171-YZ0003
IGF-1R (Tyr1131)
IR-β (Tyr1146)
IGF-1-treated HEK-293 171-YZ0005
IκB-α (Ser32/Ser36)
NF-κB p65 (Ser536)
Smad2 (Ser465/Ser467)
TNF-α-treated HeLa 171-YZ0008
p70 S6 kinase (Thr421/Ser424)
p70 S6 kinase (Thr389)
NGFβ-treated PC12 171-YZ0006
Src (Tyr416) Src-transfected NIH3T3 171-YZ0013
Stat1 (Tyr701)
Stat3 (Ser727)
Stat3 (Tyr705)
IFN-α-treated HeLa 171-YZ0004
VEGFR-2 (Tyr1175) VEGF-treated HUVEC 171-YZ0010
ZAP-70 (Tyr319) H2O2-treated Jurkat 171-YZ0012
Negative control for
all phosphoprotein assays
Phosphatase-treated HeLa 171-YZB001
Total Target Lysate Control Catalog #
Total Akt
Total Erk1/2
Total GSK-3β
Total IκB-α
Total JNK
Total MEK1
Total mTOR
Total p38 MAPK
Total p70 S6 kinase
Total PTEN
Total Smad2
Total IGF-1R
Untreated HeLa 171-YZT002
Total Btk H2O2-treated Ramos 171-YZ0011
Total ZAP-70 H2O2-treated Jurkat 171-YZ0012
Total c-Jun
Total CREB
Untreated HEK-293 171-YZT001
Total HER-2 EGF-treated SK-Br3 171-YZ0003
Total Src Src-transfected NIH3T3 171-YZ0013
Negative control for
all total target assays
Detection antibody diluent**  
Housekeeping Protein Lysate Control Catalog #
β-actin
Human GAPDH
Untreated HeLa 171-YZT002
Negative control for all housekeeping protein assays Detection antibody diluent**  

* All controls are shipped as one vial of lyophilized cell lysate.

** Detection antibody diluent is included with the Bio-Plex Pro cell signaling reagent kit (171-304006M).