EZ Load 20 bp Molecular Ruler #1708351

Image
170-8351_view.jpg

Overview

500 μl, 0.1 μg/μl, ready-to-load DNA standard, 20–1,000 bp, 50 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications
Safety Data Sheet

Safety Data Sheet

Description

Description

Use our molecular ruler to accurately assign molecular weights to DNA fragments separated by gel electrophoresis. Perfect 50% GC content eliminates spurious migration due to sequence variation, ensuring that the fragments migrate exactly according to their specified size. See Recommended Gel Types. Store product at 4°C.

Molecular
Ruler
Concentration
(µg/µl DNA)
Size Range Number of
Bands
Reference
Band
Amount
of DNA (µg)
Suggested
Loading
Volume (µl)
Number of
Applications
AmpliSize
(1708200)
0.1
(10 ng/band/µl)
50–2,000 bp 10 of
proprietary
blunt-ended
DNA
None 25 5 50
20 bp
(1708201)
0.2 20–1,000 bp 50 in 20 bp
increments
200 bp 50 2.5 100
EZ Load
20 bp

(1708351)
0.1 20–1,000 bp 50 in 20 bp
increments
200 bp 50 5 100
100 bp*
(1708202)
0.1 100–1,000 bp 10 in 100 bp
increments
None 25 2.5 100
EZ Load
100 bp
*
(1708352)
0.05 100–1,000 bp 10 in 100 bp
increments
None 25 5 100
100 bp PCR*
(1708206)
0.2 100–3,000 bp 30 in 100 bp
increments
1,000 bp &
3,000 bp
40 2 100
EZ Load
100 bp PCR
*
(1708353)
0.08 100–3,000 bp 30 in 100 bp
increments
1,000 bp &
3,000 bp
40 5 100
500 bp**
(1708203)
0.2 500–8,000 bp 16 in 500 bp
increments
5,000 bp 40 2 100
EZ Load
500 bp
**
(1708354)
0.08 500–8,000 bp 16 in 500 bp
increments
5,000 bp 40 5 100
1 kb**
(1708204)
0.2 1–15 kb 15 in 1 kb
increments
5 kb 40 2 100
EZ Load
1 kb
**
(1708355)
0.08 1–15 kb 15 in 1 kb
increments
5 kb 40 5 100
2.5 kb**
(1708205)
0.1 2.5–35 kb 14 in 2.5 kb
increments
10 kb 40 4 100

EZ Load Molecular Rulers are preblended with sample loading buffer.
The 100 bp rulers may show a double- or triple-banding pattern in polyacrylamide gels.
*The DNA fragments in this product have HindIII-compatible cohesive ends.
**The DNA fragments in this product have EcoRI-compatible cohesive ends.

For optimal resolution of <500 bp fragments, use a 3% ReadyAgarose Precast Gel or a 5% Mini-PROTEAN TBE Precast Gel. For fragments of >500 bp, use a 1% ReadyAgarose Precast Gel.

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More Information

Download the Little Book of Standards for complete product information on all Bio-Rad nucleic acid and protein standards.