Biologics Analysis Workflow

Biologics Analysis Workflow

The Biologics Analysis Workflow is a four-step solution for biological product analysis. This integrated system enables expedited electrophoresis, simplified staining, easy analysis, and data reproducibility.

Read More
Ordering Information

Overview

The Biologics Analysis Workflow is a suite of products designed and validated to assess the purity or identity of biological products in a cGMP regulatory environment. The Biologics Analysis Workflow provides:

  • Increased throughput
  • Simplified, sensitive gel staining
  • Accurate and reproducible imaging
  • Easy data analysis

The Biologics Analysis Workflow includes a set of integrated protein electrophoresis solutions for each step of the workflow:

Step 1: Separate samples

Criterion TGX precast gels — increased throughput with fast run times and more sample loading wells

The unique properties of TGX chemistry reduce run times to as little as 20 minutes. These gels have a 1-year shelf life and utilize the standard Laemmli buffer system. The midi-sized Criterion format allows you to load up to 26 samples while maintaining the required resolution. Additionally, the characteristic upper buffer chamber reduces the amount of running buffer required.

Step 2: Stain proteins

QC Colloidal Coomassie stain — highly sensitive, reliable, end-point stain designed for quantitative analysis of proteins

QC Colloidal Coomassie stain enables the detection of nanogram levels of protein. The colloidal chemistry improves the protein-to-dye binding ratio, reducing background staining and improving sensitivity. QC Colloidal Coomassie stain is an end-point stain that was designed and optimized for quantitative analysis with the GS-900 densitometer. The stain is ready to use without mixing or the addition of other reagents. The stain is formulated with ethanol instead of methanol, eliminating the need for hazardous waste disposal.

Step 3: Visualize proteins

GS-900 calibrated densitometer — reproducible imaging of protein gels, blots, and film across a wide dynamic range

To ensure the accuracy of each scan, the GS-900 calibrated densitometer contains internal optical density tablets that are scanned and used for calibration before each run. The calibration of the GS-900 densitometer can be validated with the GS-900 calibrated densitometer installation and qualification kit, which utilizes an external target to confirm accuracy of the internal target, ensuring the accuracy and reproducibility of the GS-900 densitometer. With red/green/blue tricolor CCD technology, the GS-900 calibrated densitometer enables the imaging and quantitation of film, blots, and gels stained with a wide variety of stains. The wide dynamic range permits the quantitation of proteins from 0–3.4 OD.

Step 4: Analyze results

Image Lab image analysis software — effortless analysis of samples with an intuitive, user-friendly interface

Gel analysis is greatly simplified with Image Lab software. The ability to automate analyses saves time and ensures consistent analysis from run to run. Image Lab software includes detailed tutorials and requires no previous imaging experience to produce optimum gel and blot images. The flexible system allows users to easily modify analysis settings to match SOPs and fine-tune band detection, background level, and other settings. Image Lab software makes reporting easy with the ability to create customized reports and meets regulatory requirements with an optional 21 CFR Part 11 module.

Specifications

Calibration frequency
Automatic calibration before every scan
21 CRF Part 11
Yes (optional software package)
Acquisition software
ImageLab software ≥ 5.0
Light source
LED
Wavelength
400–750 nm
Absorbance range
0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
Transmissive scan area
29 × 33 cm
Reflective scan area
29 × 33 cm
Bit depth
16-bit grayscale
Minimum pixel size
36.3 µm (700 dpi)
Image Lab™ software OS compatibility
Windows XP, 7
Connection type
USB 2.0
Imager dimensions (W x L x H)
62.7 × 37.6 × 18.5 cm
Imager weight
16.1 kg
Applications
Densitometry
Calibrated colorimetric gel and blot quantitation

Ordering

items

Use the filters below to refine results!

Image
170-7993_thumb.jpg
1707993
Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module
Image
170-7994_thumb.jpg
1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)

Kits & Reagents

Image
161-0732_tn.jpg
(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

Precast Gels

Image
567-1xxx_tn.jpg
5671023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671083

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671084

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671085

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671081

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671082

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671093

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671094

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671095

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671091

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671092

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
567-1xxx_tn.jpg
5671123

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671124

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671125

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671121

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Image
567-1xxx_tn.jpg
5671122

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

MW Standards

View All
Image
161-0373_tn.jpg
1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
Image
161-0373_tn.jpg
1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
Image
161-0374_tn.jpg
1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
Image
161-0374_tn.jpg
1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
Image
161-0377_tn.jpg
1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
Image
161-0377_tn.jpg
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
Image
161-0375_tn.jpg
1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
Image
161-0375_tn.jpg
1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
Image
161-0363_tn.jpg
1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
Image
161-0363_tn.jpg
1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Documents

Number Description Options
6385
Biologics Analysis Workflow™ Brochure, Rev A
10031959
Instruction Manual, Image Lab Software for the GS-900 Calibrated Densitometer, Rev B
10033507
Biologics Analysis Workflow Product Information Sheet
6387
Biologics Analysis Workflow™ Model Comparability Study — Obtaining cGMP Approval for Validated SDS-PAGE Assays with the GS-900™ Calibrated Densitometer
[ Add to Cart (Free) ]
6040
Electrophoresis Guide, Interactive PDF, Rev C
4006028
Instruction Manual, Laemmli Sample Buffer, Rev J
10032602
QC Colloidal Coomassie Stain Manual, Rev A
6421
Image Lab™ Software for the GS-900™ Densitometer Quick Start Guide, Rev A
10030885
Installing your GS-900™ Calibrated Densitometer Quick Start Guide, Rev C