1 X 96-well Flat Bottom Plate, Express assay format that includes coupled magnetic beads, detection antibodies, standard, detection antibody diluent, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well assay plate, sealing tape and instructions for detecting Human Th17 Cytokine IL-22, IL-23, IL-25, IL-31, IL-33, all in one kit. Analytes are mixed by user.