1x96-well filter plate, x-Plex assay format that includes premixed coupled beads, detection antibodies, and lysates for detecting phosphorylated Akt (Ser473), c-Jun (Ser63), ERK2 (Thr185/Tyr187), Histone H3 (Ser10), IGF-IR (Tyr1131), IkappaB-α(Ser32/Ser36), IRS-1 (Ser636/Ser639), JNK (Thr183/Tyr185), p38 MAPK (Thr180/Tyr182), requires phosphoprotein reagent and cell lysis kit offered separately