1x96-well filter plate, x-Plex assay format that includes premixed coupled beads, detection antibodies, and lysates for detecting phosphorylated Akt (Ser473), c-Jun (Ser63), ERK1/2 (Thr202/Tyr204, Thr185/Tyr187), GSK-3α/β(Ser21/Ser9), IGF-IR (Tyr1131), JNK (Thr183/Tyr185), p38 MAPK (Thr180/Tyr182), p70 S6 kinase (Thr421/Ser424), requires phosphoprotein reagent and cell lysis kit offered separately