1 X 96-well flat bottom plate, x-Plex assay format that includes premixed coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting rat cytokines IL-1a, IL-1ß, IL-4, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IFN-ƴ, MIP-1α, TNF-α, all in one kit.