The ProteOn liposome capturing kit includes 1 ProteOn LCP sensor chip, 1 ProteOn LCP capturing reagent kit, and the ProteOn lipid modification conditioning solution. It is capable of capturing lipid assemblies such as liposomes on a hydrophilic chip surface through DNA hybridization.
The LCP sensor chip surface is functionalized with NeutrAvidin in a planar configuration that is formed on a self-assembled monolayer. The ProteOn LCP reagent kit activates the chip surface by a biotinylated DNA tag so that the chip is able to capture DNA-labeled lipid assemblies through DNA hybridization. The reagent kit attaches DNA tags to the liposomes in order to anchor them to the chip surface. It is possible to capture two or more layers of lipid assemblies for additional sensitivity. This method of capturing allows interaction analyses involving lipid membranes or membrane proteins embedded in intact lipid assemblies.
- Hydrophilic surface for high data quality
- Low nonspecific binding for high data quality
- Good regenerability for low chip-to-chip variability and costs
- Stable capturing of lipid assemblies for high data quality
- Capturing or “building” multiple liposome layers for high sensitivity in difficult applications
- Array of 6 x 6 interactions for efficient experiment optimization and high throughput
The ProteOn liposome capturing kit includes the LCP sensor chip, the LCP capturing reagent kit required for using the sensor chip, and the lipid modification conditioning solution used to clean and stabilize the chip surface prior to capturing lipid assemblies such as liposomes. The LCP capturing reagent kit includes sufficient reagents for the use of two LCP sensor chips.