iTaq™ Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) #1725122

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1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).

  • Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument
  • Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)
  • Amplify genomic DNA and difficult amplicons
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)

Instrument Compatibility

iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 1725120
500 5 x 1 1725121
1,000 10 x 1 1725122
2,500 5 x 5 1725124
5,000 10 x 5 1725125

See the iTaq Universal SYBR Green Supermix product page for more information and documentation.

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Supporting Documents

Online Resources

Number Description Options
Available Translation
iTaq Universal SYBR Green Supermix  (SDS)gene

Bulletin# 172-5120-MSDS

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