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Description
Description
The fifth module in Cloning and Sequencing Explorer Series, the Ligation and Transmformation Module allows the ligation of PCR products into the pJET1.2 plasmid vector for transformation of competent bacteria. The kit includes T4 ligase, cloning vector, proofreading polymerase, buffers, C-Growth medium, transformation reagents, microcentrifuge tubes, and curriculum. Materials are sufficient for 12 student workstations. This product is for education use only.
Specifications
Specifications
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
T4 DNA ligase, 10 µl
1
Ligation reaction buffer, 2x, 100 µl
1
Proofreading DNA polymerase, 10 µl
1
pJET1.2 blunted cloning vector, 10 µl
1
BgIII restriction enzyme, 55 µl
1
Restriction enzyme buffer, 10x, 100 µl
1
C-Growth medium, 30 ml
1
Transformation reagent A, 5 ml
1
Transformation reagent B, 5 ml
1
IPTG ,1 M, 0.1 ml
1
Sterile water, 1 ml
1
Colored microcentrifuge tubes, 2.0 ml
120
Microcentrifuge tubes, 1.5 ml
30
Curriculum, including teacher's guide, student manual, and graphic quick guide
1