SureBeads™ Starter Kit Protein G #1614823

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Overview

Starter kit for immunoprecipitation with protein G conjugated magnetic beads, contains 2 x 3 ml protein G bead suspension (10 mg beads/ml suspension) and a 16-tube magnetic rack
Safety Data Sheet

Safety Data Sheet

Description

Description

SureBeads Starter Kits provide an ideal introduction to fast, easy, and reliable immunoprecipitation using protein A and protein G conjugated magnetic beads. The lightweight, ergonomically designed SureBeads Magnetic Rack with separable magnets enables bioseparations in seconds without centrifugation.

Kit contents:

  • 2 x 3 ml vials SureBeads Protein G Magnetic Beads; 60 mg (10 mg/ml)
  • Magnetic rack with one-piece removable magnet insert; holds 1.5 ml and 15 ml tubes
    • Up to 16 x 1.5 ml microcentrifuge tubes
    • 2 x 15 ml conical centrifuge tubes

Use SureBeads Magnetic Beads for immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays. Superparamagnetic beads with surface-activated hydrophilic polymer and chemically conjugated protein A or protein G bind the Fc regions of immunoglobulins with high specificity.

  • Faster IP using magnetization instead of centrifugation
  • Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency
  • High IgG binding affinity with low nonspecific binding and reduced antibody consumption
  • Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting

Packaging Options

Conjugated Protein Bead Suspension Volume Rack Size
(Number of Tubes)
Catalog #
G 1 ml 161-4021
G 3 ml 161-4023
G 1 ml 4* 161-4821
A 1 ml 161-4011
A 3 ml 161-4013
A 1 ml 4* 161-4811
A/G 2 x 3 ml 16 161-4833
G/G 2 x 3 ml 16 161-4823
A/A 2 x 3 ml 16 161-4813
16-tube magnetic rack 16 161-4916

*Trial kit

Related Products

More Information

Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.

Protein A/G Immunoprecipitation Guide

Monoclonal Protein A Protein G
Human IgG1 +++ +++
Human IgG2 +++ +++
Human IgG3 +++
Human IgG4 +++ +++
Mouse IgG1 + +++
Mouse IgG2a +++ +++
Mouse IgG2b ++ ++
Mouse IgG3 ++ ++
Rat IgG1 +
Rat IgG2aa +++
Rat IgG2b ++
Rat IgG2c + ++
Polyclonal Protein A Protein G
Rabbit +++ ++
Cow ++ +++
Horse ++ +++
Goat ++
Guinea pig +++ ++
Sheep +/- ++
Pig ++ ++
Rat +/- ++
Mouse ++ ++
Chicken +
Human IgG +++ +++
Human IgM +
Human IgD +
Human IgA +

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Specifications

Specifications

Bead size
2.4–3.4 µm
Bead magnetic content
14–28%
Typical IgG binding capacity
≥6 µg/mg
Solid content in slurry
1.0–1.2%
Protein A/G content
9–12 µg/mg
Dispersion medium
TBS + 0.05% Tween 20 + 0.09% sodium azide
Shelf life
Store for 24 months at 4°C until expiration date
Number of applications
1 ml vial, 10 assays; 3 ml vial, 30 assays

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