SureBeads™ Protein A Magnetic Beads, 1 ml #1614011

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Overview

1 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
Safety Data Sheet

Safety Data Sheet

Description

Description

Use SureBeads Protein A Magnetic Beads for fast, easy, and reliable bioseparations: immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays.

Superparamagnetic beads with surface-activated hydrophilic polymer and chemically conjugated protein A bind the Fc regions of immunoglobulins with high specificity.

  • Faster IP using magnetization instead of centrifugation
  • Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency
  • High IgG binding affinity with low nonspecific binding and reduced antibody consumption
  • Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting

Packaging Options

Conjugated Protein Bead Suspension Volume Rack Size
(Number of Tubes)
Catalog #
G 1 ml 161-4021
G 3 ml 161-4023
G 1 ml 4* 161-4821
A 1 ml 161-4011
A 3 ml 161-4013
A 1 ml 4* 161-4811
A/G 2 x 3 ml 16 161-4833
G/G 2 x 3 ml 16 161-4823
A/A 2 x 3 ml 16 161-4813
16-tube magnetic rack 16 161-4916

*Trial kit

Related Products

More Information

Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.

Protein A/G Immunoprecipitation Guide

Monoclonal Protein A Protein G
Human IgG1 +++ +++
Human IgG2 +++ +++
Human IgG3 +++
Human IgG4 +++ +++
Mouse IgG1 + +++
Mouse IgG2a +++ +++
Mouse IgG2b ++ ++
Mouse IgG3 ++ ++
Rat IgG1 +
Rat IgG2aa +++
Rat IgG2b ++
Rat IgG2c + ++
Polyclonal Protein A Protein G
Rabbit +++ ++
Cow ++ +++
Horse ++ +++
Goat ++
Guinea pig +++ ++
Sheep +/- ++
Pig ++ ++
Rat +/- ++
Mouse ++ ++
Chicken +
Human IgG +++ +++
Human IgM +
Human IgD +
Human IgA +

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Specifications

Specifications

Bead size
2.4–3.4 µm
Bead magnetic content
14–28%
Typical IgG binding capacity
> 6 µg/mg
Solid content in slurry
1.0–1.2%
Protein A/G content
9–12 µg/mg
Dispersion medium
TBS + 0.05% Tween 20 + 0.09% sodium azide
Shelf life
Store for 24 months at 4°C until expiration date
Number of applications
1 ml vial, 10 assays; 3 ml vial, 30 assays