S3™ Cell Sorter (488/561 nm) #1451002


The S3 Cell Sorter has been replaced by the S3e Cell Sorter. However, we will continue to provide support for customers who own the S3 Cell Sorter.



Discontinued Product
The S3 Cell Sorter has been replaced by the S3e™ Cell Sorter. However, we will continue to provide support for customers with the S3 Cell Sorter.


This automated benchtop cell sorter comes equipped with two lasers, a 488 nm, 100 mW laser; and a 561 nm, 100 mW laser; four fluorescence detectors; and forward- and side-scatter detectors. The S3 Cell Sorter uses state-of-the-art ProDrop™ Technology for automated drop delay calculation and droplet break-off monitoring, thereby simplifying one of the most complex and error-prone aspects of cell sorting.

This affordable cell sorter provides exceptional sort purity without compromising performance and sensitivity. The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, mCherry, or for cells labeled with fluorescent markers such as labeled antibodies.

Features and Benefits

  • Simplified instrument setup — automated drop delay calculation and droplet break-off monitoring enables precise 1–4 color sorting with minimal training
  • Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
  • Reduced carryover — dual-position loading stage ensures the sample line remains clean between sorting runs
  • 2-way cell sorting — sort two different defined populations at the same time
  • High speed and high purity — sort cells fast while maintaining high sensitivity and purity
  • Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition

For added biosafety during the cell sorting process, the optional S3e™ Biosafety System Class I (145-1078) is available. Custom-designed to work specifically with the S3 or S3e Cell Sorter, the S3e Biosafety System Class I provides protection for both the user and the environment from aerosols created while sorting. The S3e Biosafety System is an affordable alternative to the large and expensive traditional biosafety hoods offered for cell sorters.



Droplet frequency
37–43 kHz
Sorting type
True jet-in-air for high-performance sorting
Sorting rate
No hardware limitations for sort rate, limited only by droplet frequency and application
Sorting purity
>99% pure
Nozzle size, µm
Sorting direction
2-way sorting
Sorting collection
Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Primary: 488 nm 100 mW
Secondary: 561 nm 100 mW
Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
<125 MESF for FITC and PE
Temperature range
Sample and collection temperature control system: 4–37°C
Peltier solid-state system
Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format
FCS 3.1
Dimensions (W x D x H)
70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)