VersaFluor™ Fluorometer

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Overview

The VersaFluor fluorometer is now discontinued, but cuvettes, filters, and other accessories are still available for current users of the instrument.
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Description

Description

Discontinued Product

The VersaFluor fluorometer is no longer available. However, current users of this instrument can still order cuvettes, filters, and other accessories.

Fluorescent assay fluorometer kits, for rapidly assaying the reporter enzymes β-glucuronidase and β-galactosidase or for quantitating DNA, can be purchased for use with the VersaFluor fluorometer.

The VersaFluor fluorometer provides fluorescent detection at high levels of sensitivity and selectivity for:

  • Quantitating DNA, RNA, and proteins
  • Detecting apoptosis
  • Performing reporter enzyme assays

If you are already using the VersaFluor fluorometer, refer to this Filter Selection Guide to determine the appropriate filters for your applications.

Specifications

Specifications

Light source
20 W quartz halogen lamp, 350–900 nm
Lamp life
2,000 hr
Sensing device
Photodiode
Optical filter size
Excitation: 1" diameter with holder
Emission: 1" diameter with holder
Cuvette sizes
Standard: 12.5 x 12.5 mm (outside dimension)
Sensitivity
10 ng/ml DNA using Hoechst dye in a 2 ml assay volume
Sensitivity selection
Three setting: Low, Med, and High, with approximate factor of 10 between each setting
Dynamic range
5 orders of magnitude using all 3 sensitivity selections
Regulatory
EN61010–1, CE
Printer output
RS–232 serial port
Printer
VersaFluor thermal printer
AC power input
100–240 VAC, 50–60 Hz
Weight
4 kg
Dimensions
17 x 33 x 23 cm

Accessories

Accessories

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1702412
Pkg of 10 rolls, for use with the VersaFluor™ thermal printer (#170-2410)
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(Discontinued) Standard Cuvettes
1702415
Pkg of 100, 4-sided, optically clear polystyrene, 3.5 ml, 12.5 x 12.5 mm (outside dimension)
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(Discontinued) Microcuvette
1702416
Pkg of 100, 4-sided microcuvettes, optically clear polystyrene, 150–300 μl, 3.0 x 3.0 mm (outside dimension)

Filter Sets

Filter Sets

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1702447
Pkg of 1, set of one excitation and one emission filter for wild-type GFP and UV-excited GFP, for use with the VersaFluor fluorometer
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1702437
Pkg of 1, set of one excitation and one emission filter, for use with the VersaFluor fluorometer (#170-2402, 170-2403)
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1702436
Pkg of 1, set of one excitation and one emission filter for fluorescein assays, for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702435
Pkg of 1, set of one excitation and one emission filter for DNA quantitation (Hoescht 33258), β-galactosidase (MUG), β-glucuronidase (MUGluc) assays

Filters

Filters

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1702446
Pkg of 1, individual emission filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702426
Pkg of 1, individual emission filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702425
Pkg of 1, individual emission filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702424
Pkg of 1, individual emission filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702421
Pkg of 1, individual emission filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702444
Pkg of 1, individual excitation filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702443
Pkg of 1, individual excitation filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702423
Pkg of 1, individual excitation filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702422
Pkg of 1, individual excitation filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702427
Pkg of 1, individual excitation filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)
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1702420
Pkg of 1, individual excitation filter for use with the VersaFluor fluorometer (#170-2402, #170-2403)

Documents

Documents

Number Description Options
2431
Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2434
Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
2436
Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
2387
Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer, Rev A
2368
Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
M1702401
Instruction Manual, VersaFluor Fluorometer, Rev B
4000107
Instruction Manual, Fluoromark Microplate Fluorometer and Fluoromark Software, Rev A