TGX Stain-Free™ FastCast™ Acrylamide Solutions

TGX Stain-Free™ FastCast™ Acrylamide Solutions

TGX Stain-Free FastCast acrylamide kits for hand casting polyacrylamide gels enable fast casting, long shelf life gels, short run times, high transfer efficiency, and protein visualization in 5 minutes with stain-free technology.

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Overview

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What Is Stain-Free Technology?

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TGX Stain-Free FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels. TGX (Tris-Glycine eXtended) formulation retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. TGX Stain-Free FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems.

TGX Stain-Free chemistry enables rapid fluorescent detection of proteins directly within gels and blots with Bio-Rad's stain-free enabled imagers. Protein samples can be separated in 20 minutes and then visualized in 5 minutes.

Features and Benefits of TGX Stain-Free FastCast Acrylamide Solutions

  • Handcast gels ready to run in as little as 30 min
  • Long shelf life — handcast gels remain fresh for up to 4 weeks at 4°C
  • Stain-free visualization of proteins in your gel or blot in less than 5 min with stain-free enabled imaging systems (Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ MP, ChemiDoc XRS+, and ChemiDoc Touch Systems)
  • Electrophoresis run times as short as 20 min using the Mini-PROTEAN® Tetra or Criterion™ cells
  • Efficient protein transfers in as little as 3 min using the Trans-Blot® Turbo™ transfer system
  • Ability to monitor the success of your electrophoresis and transfer steps without additional staining

Applications and Uses of TGX Stain-Free FastCast Acrylamide Solutions

  • 1-D polyacrylamide gel electrophoresis for separation of proteins
  • Accurate molecular weight estimation
  • Robust separation for complex samples

Approximate number of gels per kit.

  Mini Gels (~9 x 7 cm) Midi Gels (~13 x 9 cm)
  Mini-PROTEAN
Cassettes		 0.75 mm
Glass Plates		 1.0 mm
Glass Plates		 1.5 mm
Glass Plates		 Criteron
Cassettes
Starter Kit 11 gels 19 gels 13 gels 9 gels 7 gels
Full Kit 55 gels 95 gels 65 gels 45 gels 35 gels

More Information

TGX FastCast tutorial video

Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide

Learn how to cast better gels with Bio-Rad's TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.

FastCast kits calculator

Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Stain-Free Imaging

Bio-Rad's V3 Western Workflow™ is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins. View the V3 Western Workflow video to learn more.

The Quick Start Guide to Stain-Free Imaging provides step-by-step instructions on how to obtain stain-free gel images from TGX Stain-Free gels in less than 5 minutes using Bio-Rad’s ImageLab™ software and stain-free enabled imagers.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.

Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Criterion and Criterion™ Dodeca™ cells use integral cassettes for gel hand casting. 1.0 mm thick gel casting sets are available in various comb configurations:

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tgx-stain-free-fastcast-acrylamide-starter-kit-sku-130-0180-thumb.jpg
(Discontinued) TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 7.5%
1610180
Starter kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
tgx-stain-free-fastcast-acrylamide-kit-sku-130-0181-thumb.jpg
(Discontinued) TGX Stain-Free™ FastCast™ Acrylamide Kit, 7.5%
1610181
Kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
tgx-stain-free-fastcast-acrylamide-starter-kit-sku-130-0182-thumb.jpg
(Discontinued) TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10%
1610182
Starter kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
tgx-stain-free-fastcast-acrylamide-kit-sku-130-0183-thumb.jpg
(Discontinued) TGX Stain-Free™ FastCast™ Acrylamide Kit, 10%
1610183
Kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
tgx-stain-free-fastcast-acrylamide-starter-kit-sku-130-0184-thumb.jpg
(Discontinued) TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 12%
1610184
Starter kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers
tgx-stain-free-fastcast-acrylamide-kit-sku-130-0185-thumb.jpg
(Discontinued) TGX Stain-Free™ FastCast™ Acrylamide Kit, 12%
1610185
Kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

Accessories

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(Discontinued) Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels
1658001FC
4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)
mini-protean-tetra-cell-165-8006fc_thumb.jpg
(Discontinued) Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.5 mm thick handcast gels
1658006FC
4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

Kits & Reagents

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(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

Standards

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(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards, value pack
1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
161-0374_tn.jpg
(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml
1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
161-0375_tn.jpg
(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack
1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
161-0377_tn.jpg
(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
161-0363_tn.jpg
(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml
1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Plates

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(Discontinued) Mini-PROTEAN® Spacer Plates with 0.75 mm Integrated Spacers
1653310
Pkg of 5, spacer plate with 0.75 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
165-3311_tn.jpg
(Discontinued) Mini-PROTEAN® Spacer Plates with 1.0 mm Integrated Spacers
1653311
Pkg of 5, spacer plate with 1.0 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
165-3312_tn.jpg
(Discontinued) Mini-PROTEAN® Spacer Plates with 1.5 mm Integrated Spacers
1653312
Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber
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(Discontinued) Mini-PROTEAN® Empty Cassettes
4560003
Pkg of 50, cassettes with 10 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0013)
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(Discontinued) Mini-PROTEAN® Empty Cassettes
4560005
Pkg of 50, cassettes with 12 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0015)

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