T100 Thermal Cycler

The 96-well T100 PCR thermal cycler offers an intuitive touch screen and reliable performance in a compact footprint.

Overview

The T100 thermal cycler is a small thermal cycler offering a comprehensive set of convenient features in a small footprint. This compact thermal cycler features an intuitive touch-screen user interface to make running PCR easy. Thermal gradient technology allows you to quickly optimize your reaction in a single run. With its robust design, the T100 system is a reliable personal thermal cycler that delivers exceptional performance for years — Launch the System Tour to test drive the T100 thermal cycler now.

Features and Benefits of the T100 Thermal Cycler

Intuitive touch screen — easy programming using a large color touch screen and intuitive interface
Consistent results — robust design ensures effective and consistent performance run to run
Easy optimization — this gradient thermal cycler allows fast PCR optimization using a unique thermal gradient
Easy protocol management — protocols can be organized using personalized folders or a USB flash drive
Small space-saving footprint — the T100 is a compact thermal cycler that fits in any laboratory

BioRad T100 Thermal Cycler Users Around the World

Life science researchers all over the world use the T100 thermal cycler in their daily routine. In this video, real-world users describe how it saves them time and helps them consistently obtain high-quality data, with high uniformity and reproducibility. The T100 thermal cycler is praised for being user-friendly, reliable, and enjoyable to use via the touch-screen interface.

Applications and Uses for the T100 Thermal Cycler

Nucleic acid amplification (PCR)
Gene cloning and analysis
Gene expression analysis
Mutational analysis
Cycle sequencing

More Information

Thermal Cycler Selection Chart

Compare the complete line of Bio-Rad PCR systems.
PCR Plastics Compatibility Chart

Find the right consumables for your instrument.

Related Technologies

Related Products

Specifications

Thermal Cycler

Sample capacity

96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate

Maximum ramp rate, °C/sec

Average ramp rate, °C/sec

2.5

Temperature range

4–100°C

Temperature accuracy

±0.5°C of programmed target

Temperature uniformity

±0.5°C well-to-well within 30 sec of arrival at target temperature

Input power

100–150 VAC, 50–60 Hz; 220–240 VAC, 50–60 Hz; 700 W maximum

Display

5.7'' VGA color touch screen

Port

1 USB A

Memory

500 typical programs; unlimited with USB flash drive expansion

Dimensions (W x D x H)

26 x 47 x 23 cm (10 x 18 x 9'')

Weight

9 kg (20 lb)

Gradient

Gradient range

30–100°C

Temperature differential range

1–25°C

Ordering

items

Use the filters below to refine results!

(Discontinued) T100 Thermal Cycler

1861096

Thermal cycler system, includes 96-well thermal cycler, power cord, tube support ring

(Discontinued) Touch-Screen Protector

1863000

Pkg of 2, optional clear plastic cover for the T100™ thermal cycler that shields the touch-screen surface from dust, reagents, and scratches

(Discontinued) Tube Support Ring

1862000

Pkg of 2, extra tube support ring for use with individual tubes in the T100™ thermal cycler

Kits & Reagents

(Discontinued) iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

1708890

25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

(Discontinued) iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns

1708891

100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

(Discontinued) iScript™ cDNA Synthesis Kit, 500 x 20 µl rxns

1708891BUN

500 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

(Discontinued) iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns

1708896

25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

(Discontinued) iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns

1708897

100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

(Discontinued) iScript™ Select cDNA Synthesis Kit, 500 x 20 µl rxns

1708897BUN

500 x 20 µl reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

(Discontinued) iTaq™ DNA Polymerase, 250 U (5 U/µl), 50 µl

1708870

250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl₂

(Discontinued) iTaq™ DNA Polymerase, 5,000 U (5 U/µl), 1 ml

1708875

5,000 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 25 ml each 10x PCR buffer and 50 mM MgCl₂

(Discontinued) MgCl₂ Solution for PCR, 50 mM, 1.25 ml

1708872

1.25 ml, 50 mM MgCl₂ solution, for PCR applications

(Discontinued) dNTP Solution Mix, 10 mM, 200 µl

1708874

200 µl, premixed dNTP solution, 10 mM each (dATP, dCTP, dGTP, dTTP)

(Discontinued) iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl

1725301

100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl₂ solution, DMSO

(Discontinued) iProof™ High-Fidelity DNA Polymerase, 500 U (2 U/µl), 250 µl

1725302

500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl₂ solution, DMSO

(Discontinued) iProof™ High-Fidelity DNA Polymerase

1725302BUN

5 x 500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl₂ solution, DMSO

(Discontinued) iProof HF Master Mix, 100 x 50 µl rxns, 2.5 ml

1725310

100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates

(Discontinued) iProof GC Master Mix, 100 x 50 µl rxns, 2.5 ml

1725320

100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates

(Discontinued) iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl

1725330

50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl₂ solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

(Discontinued) iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl

1725331

200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl₂ solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

Gel Systems

(Discontinued) Sub-Cel GT Horizontal Electrophoresis System, 15 x 15 cm tray, with gel caster and casting gates

1704482

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 15 cm (W x L) UV-transparent tray, casting gates, gel caster

(Discontinued) Sub-Cell GT Horizontal Electrophoresis System, 15 x 25 cm tray, with gel caster

1704484

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 25 cm (W x L) UV-transparent tray, gel caster

(Discontinued) Wide Mini-Sub Cell GT Horizontal Electrophoresis System, 15 x 10 cm tray

1704468

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm (W x L) UV-transparent tray

(Discontinued) Wide Mini-Sub Cell GT Horizontal Electrophoresis System, 15 x 10 cm tray, with gel caster

1704469

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm (W x L) UV-transparent tray, mini-gel caster

(Discontinued) Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray

1704466

Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray

(Discontinued) Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray, with mini-gel caster

1704467

Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm (W x L) UV-transparent tray, mini-gel caster

Plastics

(Discontinued) Multiplate™ 96-Well PCR Plates, high profile, unskirted, clear

MLP9601

Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples

(Discontinued) Hard-Shell® 96-Well PCR Plates, high profile, semi skirted, clear/clear

HSS9601

Pkg of 25, clear shell/clear well PCR plate to fit most instruments, rigid 2-component design

(Discontinued) iQ™ 96-Well PCR Plates, high profile, semi-skirted

2239441

Pkg of 25, semi-skirted PCR plate for iCycler iQ^®, iQ™5, MyiQ™, and MyiQ™2 Real-Time PCR Detection Systems

(Discontinued) Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001

Pkg of 100, optically clear seal for PCR plates. Note: This item is now available under the new material number MSB1001B. For future orders please proceed here.

(Discontinued) Microseal® 'A' PCR Plate and PCR Tube Sealing Film

MSA5001

Pkg of 50, nonadhesive sealing film for PCR plates and tubes

(Discontinued) Microseal® 'F' PCR Plate Seal, foil, pierceable

MSF1001

Pkg of 100, aluminized foil seal for PCR plates, pierceable. Note: This item is now available under the new material number MSF1001B. For future orders please proceed here.

(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, high profile, clear

TBS0201

Pkg of 125, thin-wall polypropylene 8-tube strips for PCR, natural (1,000 tubes)

(Discontinued) 0.2 ml 12-Tube PCR Strips without Caps, high profile, clear

TBS1201

Pkg of 100, thin-wall polypropylene 12-tube strips for PCR, natural (1,200 tubes)

(Discontinued) 0.2 ml Domed PCR Tube 8-Cap Strips, clear

TCS0801

Pkg of 130, 8-cap strips for 0.2 ml tubes and plates for PCR, clear

(Discontinued) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear

TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

(Discontinued) 0.2 ml Domed PCR Tube 12-Cap Strips, optical, clear

TCS1201

Pkg of 200, 12-cap strips for 0.2 ml tubes and plates for PCR, clear

(Discontinued) 0.2 ml PCR Tubes with Flat Caps, high profile, clear

TFI0201

Pkg of 1,000, thin-wall polypropylene PCR tubes with attached caps for labeling, full-height wells, natural

(Discontinued) 0.2 ml PCR Tubes with Domed Caps, high profile, clear

TWI0201

Pkg of 1,000, thin-wall polypropylene PCR tubes with attached domed caps, full-height wells, natural

Downloads

T100™ Firmware Upgrade

SOFT-T100-FW

Firmware files and instructions for the T100 thermal cycler. Version 1.201

Download Software

Software Download

EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
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All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

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Documents

Number Description Options

10020524

T100 Thermal Cycler Quick Guide, Rev B

10021426

Amplification Plastics and Reagents Selection Guide for the T100™ Thermal Cycler, Rev B

10000145577

T100 Thermal Cycler Instruction Manual

6777

Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR

6060

T100 Thermal Cycler Specifications Sheet

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LIT-6065

T100 Thermal Cycler Brochure, Rev C