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Overview

iTaq Polymerase Performance Data

Exceptional Performance

iTaq DNA Polymerase outperforms a chemically modified hot-start DNA polymerase over a wide range of targets. Human genomic DNA fragments from 300 bp to 4 kb were amplified with both polymerases. Lane M, EZ Load 500 bp molecular ruler.

Amplification of Low-Copy Targets

iTaq DNA Polymerase performs better than other polymerases, even at low target concentrations. A 510 bp target in human genomic DNA was amplified with iTaq DNA Polymerase or another supplier's hot-start DNA polymerase according to manufacturers' recommendations. Lane M, 100 bp EZ Load Molecular Ruler.

Higher Yields

The high sensitivity of iTaq DNA Polymerase translates to higher yields per unit of enzyme. Human genomic DNA was amplified with iTaq DNA Polymerase or another supplier's hot-start DNA polymerase according to manufacturer recommendations. Lane M, 100 bp EZ Load Molecular Ruler.

Includes iTaq Polymerase, an antibody-mediated hot-start DNA polymerase suitable for both PCR and real-time PCR applications, plus buffers and dNTPs.


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Description

iTaq DNA Polymerase is an antibody-mediated hot-start DNA polymerase suitable for both PCR and real-time PCR applications. The enzyme is activated after a 3 min denaturation step at 95°C. iTaq DNA Polymerase is highly specific, sensitive, and easy to use.

Features and Benefits of iTaq DNA Polymerase:

  • Improved product yields compared to non-antibody-based DNA polymerases
  • Amplification of a wide range of targets
  • Convenient reaction assembly at room temperature

Choose the best DNA polymerase for your application.

Key Features iProof DNA Polymerase iTaq DNA Polymerase
Speed
Processivity
Long fragment amplification
Proofreading Yes No
Hot-Start No Yes
 

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Supporting Documentation


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iTaq™ DNA Polymerase, 250 U (5 U/µl), 50 µl

1708870
250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2

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$287.00 
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iTaq™ DNA Polymerase, 5,000 U (5 U/µl), 1 ml

1708875
5,000 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 25 ml each 10x PCR buffer and 50 mM MgCl2

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$4,277.00 
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dNTP Solution Mix, 10 mM, 200 µl

1708874
200 µl, premixed dNTP solution, 10 mM each (dATP, dCTP, dGTP, dTTP)

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$151.00 
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MgCl2 Solution for PCR, 50 mM, 1.25 ml

1708872
1.25 ml, 50 mM MgCl2 solution, for PCR applications

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$30.00 
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Documents

Number Description Options
4106202 Instruction Manual, iTaq DNA Polymerase, Rev B Click to download
4106203 Instruction Manual, dNTP Mix, Rev A Click to download
2779 iTaq DNA Polymerase Flier, Rev E Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]

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