SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix

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Overview

Features an advanced buffering chemistry optimized for samples containing crude lysates and other PCR inhibitors. Use over a broad range of conditions on any real-time PCR system.
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Description

Description

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SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix is an exclusive high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein polymerase technology and advanced buffer formulation. This supermix is specifically formulated for SYBR Green-based qPCR with challenging samples including crude lysates and inhibitor-rich samples. This supermix delivers higher processivity, unrivaled PCR inhibitor tolerance, and robust performance across a wide range of applications on any qPCR platform and is validated to ensure optimal results with Bio-Rad’s PrimePCR Gene Expression Assays.

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix allows you to:

  • Skip RNA and DNA extraction by using crude samples — achieve superior data using crude lysates from plants, seeds, cells, bacteria, or FFPE
  • Power through PCR inhibitors — delivers unrivaled performance with common PCR inhibitors such as heparin, polysaccharides, culture media, and more
  • Increase cDNA loading — add up to 20% cDNA from our iScript Advanced cDNA Synthesis Kit for RT-qPCR or iScript Reverse Transcription Supermix
  • Obtain high-quality real-time PCR results under various experimental conditions — for example, primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)
  • Shorten time to results while maintaining qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
  • Use with any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system

Instrument Compatibility

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix

Related Products

Bio-Rad offers complete workflow solutions for real-time PCR:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patent 7,560,260

Specifications

Specifications

Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (maximum usage time) at –20ºC
12 months

Ordering

Ordering

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1725016
200 x 20 µl reactions, 2 ml (2 x 1 ml) 2x supermix, contains Sso7d fusion polymerase in an advanced buffer formulation
ssoadvanced-universal-inhibitor-172-5017-thumb.jpg
1725017
500 x 20 µl reactions, 5 ml (1 x 5 ml) 2x supermix, contains Sso7d fusion polymerase in an advanced buffer formulation
ssoadvanced-universal-inhibitor-172-5018-thumb.jpg
1725018
1,000 x 20 µl reactions, 10 ml (2 x 5 ml) 2x supermix, contains Sso7d fusion polymerase in an advanced buffer formulation

Documents

Documents

Number Description Options
10042442
SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix Product Insert, Rev A
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
6629
High-Throughput Real-Time PCR Solutions Brochure