SmartSpec Plus Spectrophotometer

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lsr_smartspec_plus.jpg

Overview

The UV/visible SmartSpec Plus spectrophotomer has a working wavelength range of 200–800 nm. It is the perfect tool for routine DNA, RNA and protein quantitation applications and simple kinetics assays.
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Description

Description

The SmartSpec Plus spectrophotometer has a wider range of features and functions than many other benchtop spectrophotometers, offering performance, stability, and functionality at an affordable price.

The UV/visible SmartSpec Plus spectrophotomer has a working wavelength range of 200–800 nm. It is the perfect tool for routine applications such as:

  • Quantitation of DNA, RNA, and oligonucleotides
  • Quantitation of proteins via the Bradford, Lowry, and BCA assay methods
  • Monitoring bacterial culture growth
  • Simple kinetic assays
  • Wavelength scans with peak detection

A simple, menu-driven interface simplifies assays and provides answers to common sample computations at the touch of a button. Conversion factors can be stored and modified. The SmartSpec Plus spectrophotometer is capable of performing calculations and providing results such as:

  • A260/A280 ratio for nucleic acid purity
  • Quantitation that takes dilution factors into account
  • Sample concentration in µg/ml (additionally in pmol/µl for oligonucleotides)
  • Molar extinction coefficient and molecular weight of oligonucleotides

At the end of an assay, a report may be printed that shows the user's identification, date, and results.

Nucleic Acid Quantitation
The SmartSpec Plus spectrophotometer offers a complete solution for the quantitation of PCR products, nucleic acid preparations, or samples to be used in bacterial transfection protocols. dsDNA, ssDNA, or RNA can be quantitated by selecting from preprogrammed conversion factors or by entering a value that is best for the sample being assayed. The instrument will provide absorbance values, concentration, and purity values so you can proceed confidently with your downstream experiments.

The SmartSpec Plus spectrophotometer also simplifies the quantitation of DNA and RNA oligonucleotides. When you enter the sequence, length, or composition, the spectrophotometer will display sample concentration in µg/ml and pmol/µl and automatically perform calculations of molar extinction coefficients and molecular weights.

Protein Quantitation
The SmartSpec Plus spectrophotometer has preprogrammed methods for the quantitation of proteins by the Bradford, Lowry, and BCA methods. Features built into each assay method facilitate data collection and present a complete analysis of assay results. The easy-to-follow menu guides you through the entire assay, ensuring that all standards and replicates are read.

  • Standards can be analyzed in groups of up to 9 replicates
  • Up to 10 standard curves can be stored under user-assigned names
  • Mean and standard deviation values are automatically calculated for each replicate group
  • Printable report includes a standard curve with r2 value

Additional Features
The SmartSpec Plus spectrophotometer also features:

  • Built-in printer
  • Xenon flash lamp extends lamp life and reduces maintenance costs
  • User interface with choice of 6 languages: English, French, German, Italian, Japanese, or Spanish
  • Spectral scans from 200 to 800 nm
  • Easy-to-use, menu-driven operation
  • Compact, space-saving design

Specifications

Specifications

Light souce
Xenon lamp
Detector
Photodiode array
Display
2 x 24 alphanumeric backlit LCD
Printer
Built-in alphanumeric/graphic printer
Interface
RS–232 serial port
Wavelength range
200–800 nm
Wavelength accuracy (at 18–27°C)
<±1.0 nm from 200 to 250, 300 to 800 nm
 
<±0.5 nm from 250 to 300 nm
Spectral bandwidth
<5 nm
Absorption accuracy
±0.01 at 0.5 AU, ±0.02 at 1.0 AU
Absorption repeatability
±0.005 at 0.5 AU
Stray light
<0.2% at 230 nm using Nal standard
Operating temperature
15–35°C
Beam height (z-dimension)
8.5 mm
Power requirement
90–260 VAC, 47–63 Hz
Regulatory
CE, TUV
Dimensions
33 x 26 x 14 cm (W x D x H)
Weight
~ 3.8 kg

Cuvettes

Cuvettes

223-9950_tn.jpg
2239950
Pkg of 100, 3.5 ml cuvette
223-9955_tn.jpg
2239955
Pkg of 100, 1.5 ml cuvette
170-2505_tn.jpg
1702505
Pkg of 1, 80–150 μl, quartz cuvette, for use with SmartSpec Plus spectrophotometer (#170-2525)
170-2504_tn.jpg
(Discontinued) Microvolume Cuvette
1702504
Pkg of 1, 200–700 μl, quartz cuvette, for use with SmartSpec Plus spectrophotometer (#170-2525)
170-2503_tn.jpg
1702503
Pkg of 1, 0.5–1.4 ml volume capacity, quartz cuvette, for use with SmartSpec Plus spectrophotometer (#170-2525)
170-2502_tn.jpg
(Discontinued) Standard Cuvette
1702502
Pkg of 1, 1–3.5 ml volume capacity, quartz cuvette, for use with SmartSpec Plus spectrophotometer (#170-2525)

Documents

Documents

Number Description Options
4006221
Instruction Manual, SmartSpec™ Plus Spectrophotometer, Rev A
4006168
Instruction Manual, SmartSpec 3000 Spectrophotometer, Rev D
2827
SmartSpec Plus Spectrophotometer Flier, Rev A.
2826
SmartSpec Plus Spectrophotometer Brochure, Rev B
5693
Measurement of low Volume DNA Samples Using the Hellma TrayCell With the SmartSpec Plus Spectrophotometer, Rev A.