Sequi-Gen GT System

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Sequi-Gen GT System

Overview

Specifications
IPC Dimensions Gel Area Minimum Buffer Volume
Recommended
Constant Power*
Expected
Voltage*
Expected
Current*
Expected
Temperature*
Upper Lower
21 x 40 cm 17 x 40 cm 500 ml 350 ml 40 W 1,600 V 25 mA 50°C
21 x 50 cm 17 x 50 cm 550 ml 350 ml 50 W 2,000 V 25 mA 50°C
38 x 30 cm 34 .5 x 30 cm 700 ml 350 ml 70 W 2,200 V 32 mA 50°C
38 x 50 cm 34 .5 x 50 cm 1,400 ml 350 ml 80 W 2,500 V 32 mA 50°C

* Typical electrophoresis conditions for a 0.4 mm, 6% acrylamide gel in 1x TBE buffer.

Simple Horizontal Gel Casting Method

Assembling and casting with the Sequi-Gen GT cell is convenient and reliable.

  1. The lever clamps slide over the gel sandwich.
  2. Each clamp exerts an even pressure over the entire length of the glass plate sandwich, resulting in a positive seal. This prevents damage to the plates or leakage, which can result from uneven pressure. Tape, screws, and binder clamps are unnecessary.
  3. With the new casting method, a precision caster is attached to the bottom of the Sequi-Gen GT cell. This assembly is placed flat on the lab bench, and the gel solution is injected with a syringe through an injection port in the precision caster.

Simple as that!

Interchangeable Sequi-Gen GT IPC sizes

Four Interchangeable Sequi-Gen GT IPC sizes.

Integral Plate Chamber

The upper buffer chamber extends over the entire area of the bonded gel plate, making contact with the top of the gel near the fill spout.

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Ever have your students sequence a gene? Now you can! The long, thin polyacrylamide gel format of the Sequi-Gen GT cell provides optimal separation of long and short DNA or RNA fragments that differ by only a single nucleotide. In addition to DNA sequencing, this system is useful for a wide array of applications:

  • Differential display
  • Microsatellite analysis
  • DNA fingerprinting and footprinting
  • Single-strand conformation polymorphism (SSCP) studies
  • Heteroduplex analysis
  • RNase protection assays
  • S1 nuclease mapping
  • Primer extension analysis
  • DNA/protein binding studies (gel shift assays)
  • Oligonucleotide analysis

The patented Sequi-Gen GT nucleic acid sequencing cell makes casting and running sequencing gels easy. The unique Sequi-Gen GT cell ensures smile-free DNA sequence and fingerprinting data and has IEC 1010 safety certification — the most rigorous international safety standard — to ensure the highest personal and environmental protection.

Key features:

  • Simple, problem-free horizontal gel casting
  • Unique design for heat dissipation maintains uniform temperature and eliminates smile patterns
  • Easy-to-use, lever-operated clamps facilitate rapid assembly and gel casting without tape and grease
  • Modular design includes a universal transparent base for all four Sequi-Gen sizes
  • Convenient drain port in the sealed upper buffer chamber allows safe and easy buffer drainage
  • Comprehensive selection of combs and spacers meet all application demands
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(Discontinued) Machined Vinyl Sharkstooth Comb

1653836EDU
Pkg of 1, 24-well sharkstooth comb, 30 cm wide and 0.4 mm thick, for use with the Sequi-Gen GT sequencing cell; education use only

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Machined Vinyl Sharkstooth Comb

1653837EDU
Pkg of 1, 0.4mm, 36 wells, 5.0 μl, 15 cm wide, use with the Sequi-Gen GT sequencing cell; education use only

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Machined Vinyl Sharkstooth Comb

1653838EDU
Pkg of 1, 0.4mm, 48 wells, 3.7 μl, 15 cm wide, use with the Sequi-Gen GT sequencing cell; education use only

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Machined Vinyl Spacer

1653812EDU
Pkg of 2, 30 cm long vinyl spacers, 0.4 mm thick, for use with the Sequi-Gen GT sequencing cell; education use only

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Machined Vinyl Spacer

1653814EDU
Pkg of 2, 40 cm long vinyl spacers, 0.4 mm thick, for use with the Sequi-Gen GT sequencing cell; education use only

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Machined Vinyl Spacer

1653816EDU
Pkg of 2, 50 cm long vinyl spacers, 0.4 mm thick, for use with the Sequi-Gen GT sequencing cell; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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