S3e Cell Sorter

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S3e Cell Sorter

Overview

S3e Cell Sorter Augmented Reality App

S3e Cell Sorter

Interested in taking an augmented reality (AR) tour to see how the S3e Cell Sorter can easily fit into your lab and help you with your research?

Available on the AppStore

  • Step 1

    Download the Bio-Rad S3e application from the app store. For the best AR experience, the application requires iOS 10.0 or later installed on your device and is compatible only with iPad Air, iPad mini 2, or newer.

  • Step 2

    Request a free full-sized S3e Cell Sorter AR tracking mat (29 x 26 in.) to be mailed to you. The tracking mat is required by the S3e application to render images and information.

  • Step 3

    Once you have a S3e AR tracking mat and the app installed on your iPad device, take a self-guided augmented reality tour of Bio-Rad's S3e Cell Sorter. Through visual recognition, simply point your app installed device at the S3e tracking mat to experience a life-sized 360° walk around the S3e Cell Sorter. Open doors and take a look inside. Place the mat on your laboratory bench to see how it can easily fit into your space.

Download the App from the AppStore  Request a Tracking Mat
Automated Drop Delay Measurement and Monitoring

Optimal Cell Sorting Performance
and Purity

Obtaining optimal sort purity and maximizing recovery requires accurate drop delay determination, or measurement of the distance between the laser point of analysis and the last connected drop.

Historically, the drop delay process has been labor intensive and has required bead counting on a microscope or image processing with a camera.

ProDrop™ Technology automates drop delay determination and accurately calculates drop delay values, resulting in less opportunity for error and leading to better recovery. The exact drop delay value is calculated by measuring the number of ProLine™ Calibration Beads in the waste stream during the QC process.

Download PDF

S3e Videos
S3e™ Biosafety System Class I

Easy access to the S3™ and S3e Cell Sorters:

 

Easy access to the S3 cell sorter

The S3e Cell Sorter is enhanced with the AutoGimbal System, which enables hands-free motorized nozzle tip alignment. Real-time automation allows walk-away cell sorting.
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Ordering Information


  • Description
  • Specifications
  • Ordering
  • Accessories
  • Download
  • Consumables
  • Documents

Description

The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts.

This compact system is equipped with either one to three lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.

Features and Benefits of the S3e Cell Sorter

  • Hands-free nozzle tip alignment — features the fully automatic AutoGimbal System, driven completely by software
  • Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training
  • Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
  • 2-way cell sorting — sort two different defined populations at the same time
  • High speed and high purity — sort cells fast while maintaining high sensitivity and purity
  • Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition

Among the many innovations of the S3e Cell Sorter, two key state-of-the-art advancements are ProDrop Technology and the AutoGimbal System:

ProDrop Technology produces extremely accurate and precise results by:

  • Automating drop delay calculation and droplet break-off monitoring
  • Allowing drop delay calculation through direct counting of bead events
  • Simplifying one of the most complex and error-prone aspects of cell sorting

The highly sophisticated AutoGimbal System automates alignment of the nozzle tip and stream to optics by:

  • Providing hands-free alignment at all points on the stream and consistency in stream position from day to day and user to user
  • Combining picomotor fine motion positioning, imaging, and response to software algorithms using camera images

The affordable S3e Cell Sorter provides exceptional sort purity, recovery, and yield without compromising performance and sensitivity.

The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry, or for cells labeled with fluorescent markers such as antibodies labeled with PE, PE tandems, and other fluorophores excited by the 405, 488, 561, and 640 nm lasers.


Specifications

Droplet frequency 37–43 kHz
Sorting type True jet-in-air for high-performance sorting
Sorting rate No hardware limitations for sort rate, limited only by droplet frequency and application
Sorting purity >99% pure
Nozzle size 100 µm
Sorting direction 2-way sorting
Sorting collection Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers Single-laser: 488 nm 100 mW
Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
Dual-laser: 488 and 561 or 640 nm 100 mW
Tri-laser: 405, 488, and 561 or 405, 488, and 640 or 488, 561, and 640 mW 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity <125 MESF for FITC and PE
Temperature range Sample and collection temperature control system: 4–37°C
Peltier solid state system
Fluidics Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format FCS 3.1
Dimensions 70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)

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S3e™ Cell Sorter (405/488/561 nm)

12007058
S3e Cell Sorter, 405, 488, and 561 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (405/488/640 nm)

12007059
S3e Cell Sorter, 405, 488, and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (488 nm)

1451005
S3e Cell Sorter, 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (488/561 nm)

1451006
S3e Cell Sorter, 488 and 561 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (488/561/640 nm)

12007052
S3e Cell Sorter, 488, 561, and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (488/640 nm)

1451008
S3e Cell Sorter, 488 and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Biosafety System Class I

1451078
Biosafety System Class I for S3™ and S3e Cell Sorters, includes HEPA filter, fan blower, base, aluminum frame, 30 mil vinyl panels with magnetic attachment

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S3e™ Cell Sorter (405/488/561 nm) with S3e Biosafety System Class I

17003806
S3e Cell Sorter, 405, 488, and 561 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (405/488/640 nm) with S3e Biosafety System Class I

17003807
S3e Cell Sorter, 405, 488, and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (488/561/640 nm) with S3e Biosafety System Class I

17003808
S3e Cell Sorter, 488, 561, and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (488 nm) with S3e Biosafety System Class I

1451029
S3e Cell Sorter, 488 nm 100 mW laser, 2 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (488/561 nm) with S3e Biosafety System Class I

1451030
S3e Cell Sorter, 488 nm and 561 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (488/640 nm) with S3e Biosafety System Class I

1451032
S3e Cell Sorter, 488 nm and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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Accessories

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list price. If the problem persists, please call customer service at or send an
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S3e™ Sheath Cap Assembly, White

1441001
Pkg of 1, white sheath bottle cap assembly with quick disconnect and inline filter, for use with the S3e Cell Sorter

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$293.00 
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S3e™ DI Water Cap Assembly, Blue

1441002
Pkg of 1, blue DI water bottle cap assembly with quick disconnect and inline filter, for use with any S3™ or S3e Cell Sorter

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$293.00 
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S3e Waste Cap Assembly, Red

1441003
Pkg of 1, red waste bottle cap assembly with quick disconnect, for use with the S3e Cell Sorter

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$293.00 
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S3e™ Filter Block A

1441005
Pkg of 1, empty filter block A with 2 empty dichroic filter holders and 3 retaining rings, for use with any S3™ or S3e Cell Sorter

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$985.00 
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S3e™ Filter Block B

1441006
Pkg of 1, empty filter block B with 2 empty dichroic filter holders and 2 retaining rings, for use with any S3™ or S3e Cell Sorter

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$985.00 
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S3e™ Individual Filter Holder

1441007
Pkg of 1, empty filter holder with retaining ring, for use with any S3™ or S3e Cell Sorter

List Price: 
 
$117.00 
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Neutral Density Filter 1.0

1441008
Pkg of 1, neutral density filter 1.0, for use with any S3™ or S3e™ Cell Sorter

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$93.00 
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S3e™ Nozzle Tip, 100 µm

1441009
Pkg of 1, nozzle tip, 100 µm, for use with any S3™ or S3e Cell Sorter

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$698.00 
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S3e™ Nozzle O-Ring with Alignment Disk

1441010
Pkg of 1, includes 2 nozzle O-rings and 2 alignment disks, for use with any S3™ or S3e Cell Sorter

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$67.00 
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S3e™ Collection Adaptor Set

1441011
Pkg of 1, collection adaptor set, includes 1 right and 1 left holder to accommodate microscope slides and flat bottom 8-tube strips, for use with any S3™ or S3e Cell Sorter

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$338.00 
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S3e™ 8-Tube PCR Strip Collection Adaptor Set, low profile

12005068
Pkg of 1, collection adaptor set, includes 1 right and 1 left holder to accommodate low profile 8-tube PCR strips, for use with any S3™ or S3e Cell Sorter

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$338.00 
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2 mm Hex Driver

1441012
Pkg of 1, 2 mm hex driver, for use with any S3™ or S3e™ Cell Sorter

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$9.00 
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S3e™ Individual Dichroic Filter Holder

1441013
Pkg of 1, empty dichroic filter holder with retaining ring, for use with any S3™ or S3e Cell Sorter

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$117.00 
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S3e™ Accessory Kit

1451065
Accessory kit, includes 100 µm nozzle tip, neutral density filter 1.0, spanner wrench, 2 mm hex driver, 1 ml syringe, O-ring, and alignment disk, for use with any S3™ or S3e Cell Sorter

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$1,160.00 
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(Discontinued) S3e™ Power Conditioner

1451076
220–240 V, power conditioner for uninterruptible power supply, protects against power surges and outages, for use with any S3™ or S3e Cell Sorter

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) S3e™ Power Conditioner

1451077
100–120 V, power conditioner for uninterruptible power supply, protects against power surges and outages, for use with any S3™ or S3e Cell Sorter

This product is no longer available. Please contact technical support for more information on replacement product.

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ProSort™ Software, Version 1.6, PC (Windows 7 Pro and 10)

Version-16-PC-Windows-7-Pro-and-10
S3e Cell Sorter instrument software. Version 1.6, for PC only (Windows 7 Pro and 10).

Installation instructions for existing users:

  • Users on V1.5 can go directly to V1.6 installation
  • Users on V1.4 will download and install V1.5 first (from web), before V1.6 upgrade
  • For all other ProSort versions, please contact Bio-Rad technical support

ProSort™ Software, Version 1.5, PC (Windows 7 Pro and 10)

Version-15-PC-Windows-7-Pro-and-10
S3e Cell Sorter instrument software. Version 1.5, for PC only (Windows 7 Pro and 10).


Consumables

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list price. If the problem persists, please call customer service at or send an
email to

ProFlow Cell Filters, sterile, 30 µm, pack of 100

12012576
Pkg of 100, sterile, cup fitting, and disposable cell filters, 30 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.

List Price: 
 
$510.00 
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ProFlow Cell Filters, sterile, 50 µm, pack of 100

12012574
Pkg of 100, sterile, cup fitting, and disposable cell filters.Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.

List Price: 
 
$510.00 
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ProFlow Cell Filters, sterile, 70 µm, pack of 100

12012573
Pkg of 100, sterile, cup fitting, and disposable cell filters, 70 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.

List Price: 
 
$510.00 
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ProLine™ Universal Calibration Beads

1451086
15 ml (3 x 5 ml), ready-to-use universal fluorescent beads for alignment verification and drop delay determination, for use on any S3™ or S3e™ Cell Sorter

List Price: 
 
$608.00 
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ProLine™ Rainbow Beads

1451085
5 ml (1 x 5 ml), include 3.0–3.4 µm beads dyed with 8 different fluorescent intensities for testing sensitivity and linearity on any S3™ or S3e™ Cell Sorter

List Price: 
 
$540.00 
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(Discontinued) ProLine™ Calibration Beads

1451081
Discontinued Product
This item has been replaced by ProLine™ Universal Calibration Beads #145-1086

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) ProFlow Sort Grade 8x Sheath Fluid

1451082
This product has been replaced by catalog #12012932.
There is no difference in unit volume or composition between the discontinued product and the replacement product.

This product is no longer available. Please contact technical support for more information on replacement product.
ProFlow Sort Grade 8x Sheath Fluid

12012932
20 L (5 x 4 L), ready-to-use, sterile endotoxin- and preservative-free sheath fluid generates 160 L of 1x solution, for use on any S3 or S3e Cell Sorter

List Price: 
 
$525.00 
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ProFlow™ Sort Grade Water

1451083
20 L (5 x 4 L), ready-to-use, sterile endotoxin-free water in gamma-irradiated S3e™ Fluidic Containers, for use on any S3™ or S3e Cell Sorter

List Price: 
 
$456.00 
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S3e™ Fluidic Container

1451084
3 x 4 L, gamma-irradiated containers, for use on any S3™ or S3e Cell Sorter

List Price: 
 
$90.00 
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Documents

Number Description Options
7187 High-Speed Sorting of Rare Cell Subsets with High Purity and Viability Using the S3e Cell Sorter Click to download
7074 S3e™ Cell Sorter Flier Click to download [ Add to Cart (Free) ]
6614 S3e™ Cell Sorter Brochure Click to download [ Add to Cart (Free) ]
7261 Top 50 Journal Publications for the S3e Cell Sorter Click to download [ Add to Cart (Free) ]
10032007 S3e™ Cell Sorter Quick Guide Click to download
10031105 Instruction Manual, S3™ and S3e™ Cell Sorters Click to download
7064 S3e Cell Sorter Laser Configuration Guide Click to download
6404 S3e™ Biosafety System Class I Flier Click to download
6503 S3e™ Biosafety System Class I Brochure Click to download [ Add to Cart (Free) ]
10030825 ProFlow™ Sort Grade 8x Sheath Fluid Product Insert Click to download
10032248 ProFlow™ Sort Grade Water Product Insert Click to download
10033040 ProLine™ Rainbow Beads Product Insert Click to download
10041517 ProLine™ Universal Calibration Beads Product Insert Click to download
10033232 Instruction Manual, S3™ Biosafety System Class I Click to download
10041735 S3e™ Filter Block (A or B) with Dichroic Filter Holders Product Insert Click to download
10041736 S3e™ Individual Filter Holder Product Insert Click to download
7055 Identifying Heterogeneity within Rare Cell Populations by Pairing Single-Cell RNA-Seq with Cell Sorting Click to download
6806 Uncovering Actionable Correlations in Heterogeneous Cell Populations for Drug Discovery and Development Click to download
6688 Sorting Bone Marrow Mesenchymal Stem Cells with the S3™ Cell Sorter Application Note Click to download
10041737 S3e™ Individual Dichroic Filter Holder Product Insert Click to download
6609 Isolation of Stably Transfected Fluorescent Cells with the S3™ Cell Sorter Application Note Click to download
6853 Reliable Single-Cell Sorting with Bio-Rad's S3e™ Cell Sorter Application Note Click to download
6681 Discriminating Copy Number Variation from Heterogeneous Samples Using the S3™ Cell Sorter and the QX200™ Droplet Digital™ PCR System Application Note Click to download
7058 S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488 nm laser) Click to download
7059 S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488/561 nm laser) Click to download
7060 S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488/640 nm laser) Click to download
7061 S3e Cell Sorter Fluorophore and Dye Compatibility Chart (405/488/561 nm laser) Click to download
7062 S3e Cell Sorter Fluorophore and Dye Compatibility Chart (405/488/640 nm laser) Click to download
7063 S3e Cell Sorter Fluorophore and Dye Compatibility Chart (488/561/640 nm laser) Click to download
6705 S3e™ Cell Sorter Daily Activities Guide Click to download
6777 Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR Click to download
N6708 5 Tips to Secure Funding for a Flow Cytometer or Cell Sorter Click to download
10000121934 Product Information sheet, ProFlow Cell Filters for Flow Cytometry Click to download

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