Reliance Select cDNA Synthesis Kit

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Overview

Use the Reliance Select cDNA Synthesis Kit to capture GC-rich targets and secondary structure in FFPE and difficult samples with degraded RNA or inhibitors.

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Description

Description

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Reliance Select cDNA Synthesis Kit contains a newly engineered reverse transcriptase designed to perform well with challenging samples and targets. This kit has the capability of processing samples with difficult inhibitors and degraded RNA (e.g., FFPE samples) as well as targets containing secondary structures and/or high GC content.

Reliance Select cDNA Synthesis Kit:
Overcome Challenging Samples

Features and Benefits

  • Novel engineered reverse transcriptase with superior performance — compatible with degraded samples such as FFPE, inhibitors and targets with high GC content
  • 10 minute reaction protocol — quickly and easily complete cDNA synthesis
  • High tolerance to common reaction inhibitors — enables higher reproducibility and consistent results, even in samples with inhibitor carry-over
  • High versatility — stock a single reverse transcription kit in the lab
  • Thermostability up to 55ºC allows for better performance with secondary structures

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Specifications

Specifications

Storage at –20°C
Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

Ordering

Ordering

items

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12012802
25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
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12012801
100 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
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17006111
500 x 20 µl reactions; bundle of 5 boxes of 100 reaction kit; includes 2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water

Accessories

Accessories

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HSP9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design
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HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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MSB1001
Pkg of 100, optically clear seal for PCR plates
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TLS0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)
TCS0803
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

Kits & Reagents

Kits & Reagents

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1725270
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions
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1725280
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions

Documents

Documents

Number Description Options
7330
Reliance Select cDNA Synthesis Kit Product Information Sheet
10000124806
Reliance Select cDNA Synthesis Kit User Protocol
6090
Amplification Reagents and Plastics Brochure
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