Reliance Select cDNA Synthesis Kit

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Reliance Select cDNA Synthesis Kit

Overview

Overcome Challenging Samples
Unrivaled Performance in the Presence of Inhibitors
Inhibitor Performance

The Reliance Select cDNA Synthesis Kit demonstrates an efficiency superior to that of the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific Inc. #18091200) in the presence of inhibitors. Varying amounts of Cervical Adenocarcinoma (HeLa-S3) Total RNA (Thermo Fisher Scientific Inc. #AM7852), ranging from 1 pg to 2 μg, were used in a Reliance Select Kit () or a SuperScript IV System () reaction with oligo(dT)20 according to the corresponding protocols. One of each duplicate reaction was spiked with a known PCR inhibitor. First-strand cDNA libraries were used in qPCR reactions using the SsoAdvanced SYBR® Green Supermix and an assay panel that included high- and low-expressing targets, GC-rich amplicons, and amplicons of varying lengths. The Reliance Select Kit delivers more consistent results and earlier average Cq (quantification cycle) than the SuperScript IV System, as is evident from the lower ΔCq values of treated versus untreated reactions. Cqs in the SuperScript IV System reactions were delayed (up to a 3-Cq delay with formalin) in the presence of most inhibitors, whereas Reliance Select Kit reactions were within 0.5 Cq of their controls.

 
Overcome Challenging Samples
Efficiency

The Reliance Select Kit delivers earlier Cqs (quantification cycles) compared to the SuperScript IV System when tested with artificially degraded RNA samples. Varying amounts of degraded Cervical Adenocarcinoma (HeLa-S3) Total RNA ranging from 1 ng to 1 μg, were reverse transcribed according to the product protocols, using the Reliance Select Kit () or the SuperScript IV System () reactions and random primers. First-strand cDNA libraries were used in qPCR reactions with the SsoAdvanced SYBR® Green Supermix and an assay panel that included common reference gene targets. The average Cq across the concentration range for a given target using degraded RNA was compared to the average Cq of duplicate reactions using intact RNA. The Reliance Select Kit delivered more accurate results between intact and degraded RNAs than the SuperScript IV System, as is evident from the lower ΔCq values.

Unbiased Reverse Transcription and Superior Sensitivity
Sensitivity

Reliance Select Kit allows for sensitive detection of low-abundance targets. ERCC RNA Spike-In Mix controls (Thermo Fisher Scientific #4456740) were reverse transcribed in the presence or absence of background RNA (1 μg of Cervical Adenocarcinoma [HeLa-S3] Total RNA) using random primers and the Reliance Select Kit or SuperScript IV System according to the product protocols. First-strand cDNA libraries were used in qPCR reactions targeting ERCC-0034. The low-abundance ERCC spike-ins in the presence of background RNA were more sensitively detected with the Reliance Select Kit. This is evident when comparing the <1 Cq shift between samples with and without 1 μg background RNA when transcribed with the Reliance Select Kit to the >3 Cq delay when transcribed with the SuperScript IV System. RFU, relative fluorescence units.

Use the Reliance Select cDNA Synthesis Kit to capture GC-rich targets and secondary structure in FFPE and difficult samples with degraded RNA or inhibitors.


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Description

CFX Loyalty Program

Earn a free one-year service contract on your CFX System with purchase of reagents.

CFX Loyalty Program

Reliance Select cDNA Synthesis Kit contains a newly engineered reverse transcriptase designed to perform well with challenging samples and targets. This kit has the capability of processing samples with difficult inhibitors and degraded RNA (e.g., FFPE samples) as well as targets containing secondary structures and/or high GC content.

Features and Benefits

  • Novel engineered reverse transcriptase with superior performance — compatible with degraded samples such as FFPE, inhibitors and targets with high GC content
  • 10 minute reaction protocol — quickly and easily complete cDNA synthesis
  • High tolerance to common reaction inhibitors — enables higher reproducibility and consistent results, even in samples with inhibitor carry-over
  • High versatility — stock a single reverse transcription kit in the lab
  • Thermostability up to 55ºC allows for better performance with secondary structures

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Specifications

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

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Reliance Select cDNA Synthesis Kit, 25 x 20 µl reactions

12012802
25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water

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$271.00 
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Reliance Select cDNA Synthesis Kit, 100 x 20 µl reactions

12012801
100 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water

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$817.00 
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Reliance Select cDNA Synthesis Kit, 500 x 20 µl reactions

17006111
500 x 20 µl reactions; bundle of 5 boxes of 100 reaction kit; includes 2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water

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$3,677.00 
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Accessories

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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

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$276.00 
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$298.00 
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Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001
Pkg of 100, optically clear seal for PCR plates

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$191.00 
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0.2 ml 8-Tube PCR Strips without Caps, low profile, white

TLS0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

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$130.00 
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0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear

TCS0803
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

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$43.00 
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Kits & Reagents

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SsoAdvanced Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725270
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

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$185.00 
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SsoAdvanced Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725280
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions

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$180.00 
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Documents

Number Description Options
7330 Reliance Select cDNA Synthesis Kit Product Information Sheet Click to download
10000124806 Reliance Select cDNA Synthesis Kit User Protocol Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]

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