ReadyPrep™ 2-D Cleanup Kit

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Overview

The ReadyPrep 2-D cleanup kit uses selective precipitation to remove ionic contaminants such as detergents, lipids, and phenolic compounds from protein samples prior to electrophoresis.
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Description

Description



The ReadyPrep 2-D cleanup kit uses selective precipitation to remove ionic contaminants such as detergents, lipids, and phenolic compounds from protein samples. These contaminants may interfere with separation, particularly during IEF and 2-D separations. Use of the kit improves 2-D resolution and reproducibility. This kit can also be used to clean up samples prior to other applications in which contaminants may negatively impact results.

The ReadyPrep 2-D cleanup kit:

  • Employs a TCA-like protein precipitation to remove ionic contaminants from protein samples
  • Enables concentration of proteins from samples that are too dilute, allowing for higher protein loads that can improve spot detection
  • Can be used with virtually any protein sample
  • Reduces streaking, background staining, and other gel artifacts associated with ionic contaminants
  • Provides reagents for either 5 or 50 preparations

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1632130
50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein
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1632140

5 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 5 x 100 µl protein samples each containing up to 500 µg protein

Documents

Documents

Number Description Options
4110143
Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A
3096
Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
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