QX100™ Droplet Digital™ PCR System

Description

Description

Discontinued Product
The QX100 system has been replaced by the new QX200™ Droplet Digital™ PCR system. However, we will continue to provide support for customers with the QX100 system.

 

The QX100 Droplet Digital PCR (ddPCR™) system provides an absolute quantification of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. The award-winning QX100 Droplet Digital PCR system is the third generation of PCR technology and provides a revolutionary approach to target DNA quantification.

The QX100 Droplet Digital PCR system's high-level performance lets you:

  • Detect rare DNA target copies with unmatched sensitivity
  • Measure small fold differences with the highest precision

Droplet Digital PCR Technology and Workflow

The QX100 system consists of two components: the QX100 droplet generator and the QX100 droplet reader. A Bio-Rad thermal cycler equipped with a 96–deep well reaction module is also needed to complete the Droplet Digital PCR workflow.

The QX100 droplet generator partitions samples containing cDNA or RNA template into 20,000 nanoliter-sized droplets. After PCR, droplets from each sample are streamed in single file through the QX100 droplet reader. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form.

Watch this video describing how the QX100 Droplet Digital PCR system works.

Applications and Uses for the QX100 Droplet Digital PCR System

There are many research areas that can benefit from digital PCR technology, including HIV research, cancer studies, and psychological disorders. The applications enabled by the ability of Droplet Digital PCR technology to achieve absolute quantification are described in numerous scientfic journal publications, conference poster presentations, our own BioRadiations articles, and Bio-Rad technical reports.

Watch our researcher testimonial video and read selected researcher profiles to discover how scientists in diverse fields apply digital PCR technology using the QX100 Droplet Digital PCR system. These researchers and others are using the QX100 system to help solve research problems in a variety of applications including, but not limited to, the following:

  • Copy number variation (CNV)
  • Rare sequence detection
  • Mutation detection
  • Gene expression analysis of rare transcripts
  • miRNA analysis
  • Next-generation sequencing sample quantification

A Simple, Elegant Workflow

QX100 Droplet Digital PCR system workflow

View the complete QX100 system workflow.

More Information

The QX100 Droplet Digital PCR system requires the use of either the C1000 Touch™ or S1000™ thermal cycler with 96–deep well reaction module.

Bio-Rad offers exclusive accessories and PCR reagent kits for use with the ddPCR system. PCR reagent kits include 2x supermix kits with probe-based target detection and one-step RT-ddPCR kits (with probes). The PX1™ PCR plate sealer effectively seals PCR plates prior to thermal cycling. The pierceable foil heat seal provides a secure sealing option for digital PCR applications.

Explore the features of the QX100 system in more depth in the QX100™ Droplet Digital™ PCR System Brochure (bulletin 6237) or contact your Bio-Rad sales representative for more information.

 

Specifications

Specifications

Starting sample size, µl
20
QX100 droplet generator capacity, samples/cartridge
8
Droplets generated per sample
20,000
Target copies per droplet
Up to 5
QX100 droplet reader capacity
96-well PCR plate
QX100 droplet reader throughput, wells/hr
32
Sample illumination
Light-emitting diodes
Sample detection
Photomultiplier tubes
Detection channels
FAM, HEX (VIC)
Linear dynamic range
5 orders of magnitude
Precision
±10%
Droplets per 96-well plate, million
1.4

Documents

Documents

Number Description Options
6237
QX100 Droplet Digital PCR System Brochure, Rev C
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10026322
Instruction Manual, QX100™ Droplet Generator, Rev A
10026321
Instruction Manual, QX100™ Droplet Reader and QuantaSoft™ Software, Rev A
10040717
QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev C
10040718
Droplet Generation Oil for Probes Product Insert
6782
ddPCR™ Multiplex Mutation Screening Kits Flier, Ver D
[ Add to Cart (Free) ]
6338
Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A
[ Add to Cart (Free) ]
10026235
ddPCR™ Supermix for Probes Product Insert
10026868
ddPCR™ Supermix for Probes (No dUTP) Product Insert
10033630
ddPCR Library Quantification Kit for Ion Torrent Product Insert, Rev B
6250
One-Step RT-ddPCR Kit for Probes Flier, Rev A
10028782
One-Step RT-ddPCR Kit for Probes Product Insert, Rev B
6402
ddPCR Library Quantification Kit for Illumina TruSeq Flier, Rev B
10031986
ddPCR Library Quantification Kit for Illumina TruSeq Product Insert, Rev C
6490
ddPCR Library Quantification Kit for Ion Torrent Flier, Rev A
6721
ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A
10033630
ddPCR Library Quantification Kit for Ion Torrent Product Insert, Rev B
6257
PX1 PCR Plate Sealer and Heat Sealing Consumables Brochure, Rev A
[ Add to Cart (Free) ]
10023997
Instruction Manual, PX1 PCR Plate Sealer, Rev A
6290
PrimePCR Assays and Panels Brochure, Rev D
6512
PrimePCR Assays for Droplet Digital PCR Brochure, Rev A
[ Add to Cart (Free) ]
6407
Droplet Digital PCR Applications Guide
6554
Droplet Digital PCR: Detection of DNA Methylation Application Note, Rev A
6541
Droplet Digital™ PCR: TRAPing Telomerase Activity Using Droplet Digital PCR Application Note, Rev A
6495
Bio-Rad’s ddPCR™ Library Quantification Kit for Ion Torrent Enables Accurate Library Quantification and Excellent Balancing of Pooled Libraries, Rev A
6277
Probing Copy Number Variations Using Bio-Rad's QX100 Droplet Digital PCR System, Rev A
6343
QX100 Droplet Digital PCR System for Accurate Quantification of Illumina TruSeq Libraries, Rev B
6424
Droplet Digital™ PCR: Ultra-Sensitive Rare Event Detection Using Bio-Rad’s QX100™ Droplet Digital PCR System Application Note, Rev B
[ Add to Cart (Free) ]
6475
Droplet Digital PCR: High-Resolution Copy Number Variation Analysis Application Note, Rev A
[ Add to Cart (Free) ]
6497
Droplet Digital PCR: Linking Microbial Physiology and Phylogeny Using Bio-Rad’s QX100 Droplet Digital PCR System Application Note, Rev A
6451
Droplet Digital PCR: Guidelines for Multiplexing Using Bio-Rad’s QX100 Droplet Digital PCR System Application Note, Rev A
6578
Droplet Digital PCR: Multiplex Detection of KRAS Mutations in Formalin-Fixed, Paraffin-Embedded Colorectal Cancer Samples Application Note
[ Add to Cart (Free) ]
6679
Droplet Digital PCR: Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples Application Note, Ver B
6628
Rare Mutation Detection Best Practices Guidelines
10048730
PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A
10048259
ddPCR Supermix for Residual DNA Quantification Product Insert