Profinity eXact Purification and Tag Cleavage Consumables

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Overview

Consumables include Profinity eXact purification resin, Profinity eXact mini spin columns, Bio-Scale Mini Profinity eXact cartridges, and the Profinity eXact expression and purification starter kit.
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Description

Description

Profinity eXact consumables include Profinity eXact purification resin, Profinity eXact mini spin columns, Bio-Scale Mini Profinity eXact cartridges, and the Profinity eXact expression and purification starter kit.

Profinity eXact Purification Resin

The Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. Purity of the eluted protein using this purification resin is typically higher than that for other affinity-tag systems. This is due to the specific recognition of subtilisin for its prodomain sequence. The resin can be packed into different column formats, including low- to medium-pressure columns, gravity-flow columns, and mini spin columns, offering users added purification flexibility.

Additional advantages of Profinity eXact purification resin include:

  • Purification and processing of fusion-tagged proteins in a single-step
  • On-column cleavage in as little as 30 min
  • No protease addition required
  • Precise cleavage at N-terminus to generate native protein sequence

The resin is supplied in 100 mM sodium phosphate, pH 7.2, containing 0.02% sodium azide. It is also available prepacked in 1 ml and 5 ml cartridges, and in mini spin columns.

Profinity eXact Mini Spin Columns

Profinity eXact mini spin columns are ideal for rapid expression screening and optimization of affinity-tag purification protocols. These single-use columns contain 0.1 ml settled Profinity eXact resin and all necessary plasticware for binding, washing, and elution steps. Profinity eXact mini spin columns have been designed to fit in all major benchtop centrifuges. The mini spin columns are available separately or in kits:

  • Profinity eXact mini spin column ten-packs (each column contains 0.1 ml settled resin) with collection tubes for binding, washing, and elution steps
  • Profinity eXact mini spin purification starter kit (includes ten mini spin columns, collection tubes, control protein lysate, bacterial lysis reagent, bind/wash buffer, and elution buffer)
  • Profinity eXact expression and purification starter kit (includes Profinity eXact mini spin purification starter kit and Profinity eXact cloning and expression starter kit)

Bio-Scale Mini Profinity eXact Cartridges

These 1 ml and 5 ml cartridges can be used with BioLogic chromatography systems, the Profinia protein purification system, a peristaltic pump, or with appropriate column adaptors with any other chromatography system. These cartridges are prepacked with Profinity eXact purification resin.

Profinity eXact Expression and Purification Starter Kit

The Profinity eXact expression and purification starter kit can be used to easily evaluate Profinity eXact fusion-tag technology and is suitable for new purifications requiring tag removal and for purifications where cleavage has resulted in inferior results. The Profinity eXact expression and purification starter kit includes two kits: the Profinity eXact cloning and expression starter kit for the cloning and expression of a target gene using pPAL vectors and competent cells, and the Profinity eXact mini spin purification starter kit for single-step purification and on-column cleavage of the tagged protein.

Specifications

Specifications

Functional ligand
Subtillisin protease, 27.8 kD
Base bead
6% agarose bead
Form
50% suspension in 100 mM sodium phosphate, pH 7.2, 0.02% sodium azide
Practical size range
60–160 µm
Dynamic binding capacity
>3 mg tag-free protein/ml resin
Recomended linear flow rate
1,000 cm/hr at 25°C
pH stability
2–13
Chemical compatibility
Common reagents including detergents, reducing agents, buffering agents, and additives.
Storage
4°C
Shelf life in 20% ethanol
>1year at 4°C
Operational Temperature
4–40°C

Ordering

Ordering

items

Use the filters below to refine results!

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7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
732-4648_tn.jpg
7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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1563008
Kit includes 1 Profinity eXact cloning and expression starter kit (20 reactions) and 1 Profinity eXact mini spin purification starter kit (10 spin columns)
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1563006
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers
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12009292
Pkg of 1, 1 ml, prepacked affinity column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009293
Pkg of 5, 1 ml, prepacked affinity columns, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009294
Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
[ Add to Cart (Free) ]
6334
Tips and Tricks for Successful Preparative Chromatography and Protein Purification, Rev B
5652
Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A
5656
Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B
5668
Profinity eXact Fusion-Tag System Brochure, Rev A
5646
Profinity eXact™ Cloning and Expression Kits, PIS, Rev B
5655
Profinity eXact™ Resin Product Information Sheet, Rev A
5811
Profinity eXact Fusion-Tag System FAQs, Rev A
5898
Using the Profinity eXact Fusion-Tag System: Strategies for Success, Rev A
10011161
BL21(DE3) Chemi-Competent Expression Cells Manual, Rev A
10011164
Bio-Scale Mini Profinity eXact™ Cartridges Manual, Rev B
10011260
Profinity eXact Protein Purification System Manual, Rev A
5772
Periplasmic Expression and Purification of Recombinant Proteins from E.coli Using the Profinity eXact Fusion-Tag System, Rev A
5928
Application of the Profinity eXact Fusion-Tag System to Eukaryotic Expression Systems Tech Note, Rev B