Precision Melt Analysis™ Software

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Precision Melt Analysis™ Software

Overview

SNP Genotyping
 

SNP genotyping by Precision Melt Analysis software using data generated by the CFX96 real-time PCR detection system. Discrimination of human factor V coagulation SNP genotypes (C to T substitution) using SsoFast EvaGreen supermix. Data from homozygous wild type (), mutant (), and heterozygote () samples are shown on a normalized melt curve plot. RFU, relative fluorescence units.

Difference Curve Plot
 

A representative difference curve plot generated from data normalized to a mutant sample. Data from homozygous wild type (), mutant (), and heterozygote () samples are shown. RFU, relative fluorescence units.

Melt Curve Plot
 

Multifile melt study analysis. Compare melt curves from different files using the Melt Study module in Precision Melt Analysis software. RFU, relative fluorescence units.

qPCR Applications Guide
qPCR Applications Guide

The qPCR Applications Guide is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:

  • Key concepts of real-time PCR
  • Getting started — experimental design
  • Multiplexing considerations
  • Data analysis
  • Gene expression analysis
  • Genotyping allelic discrimination
  • GMO detection

This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.

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Precision Melt Analysis software imports and analyzes data files generated from all CFX real-time PCR detection systems to genotype samples based on the thermal denaturation properties of double-stranded DNA. The software can be used for a variety of genotyping applications, including scanning for new gene variants, screening DNA samples for SNPs, identifying insertions/deletions or other unknown mutations, and determining the percentage of methylated DNA in unknown samples. Use the default analysis settings to automatically normalize data and assign a genotype to each sample based on its melt characteristics — there is no need to include genotype controls to assign cluster labels.

Precision Melt Analysis software saves analysis time by assigning sample genotypes automatically based on cluster analysis, or manually using multiple data view options to tailor the software to the appropriate analysis. Use the normalized melt curves plot feature to generate a basic representation of the different clusters based on curve shifting (for homozygotes) and curve shape change (for heterozygotes). Difference curve plots of a sample fluorescence versus a selected control at each temperature transition provide a convenient visual aid to interpret the data.

Precision Melt Analysis software enables data comparison between multiple file runs by combining data into a single Melt Study. Develop a standard library of melt curve runs to analyze an unlimited number of melt experiments without having to export data.

Precision Melt Analysis software makes it easy for you to:

  • Streamline your data analysis using the customizable default analysis settings
  • Utilize the multiple data view options to manually assign sample genotypes by tailoring the software to the appropriate analysis
  • Examine results from a number of melt files, without having to export data, using the Melt Study module
  • Analyze multiple experiments from a single plate using the Well Groups feature
  • Publish your data in several formats by easily exporting data to Microsoft Excel or as an image
  • Precision Melt Analysis Software is compatible with Windows 7, 8, or 10

 

Operating system Minimum: Windows 7
Recommended: Windows 10
Drive Minimum: CD-ROM
Recommended: CD-RW
Downloadable version is available
Hard drive Minimum: 10 GB
Recommended: 20 GB
Processor speed Minimum: 1 GHz
Recommended: 2 GHz
RAM 2 GB
Screen resolution Minimum: 1,024 x 768 with true-color mode
Recommended: 1,280 x 1,024 with true-color mode
USB port 2.0 Hi-Speed or above
Software Microsoft Office Suite
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Precision Melt Analysis Software

1845025
2 user licenses, data analysis software for all CFX real-time PCR detection systems, 2 HASP HL keys, calibration kit, installation CD.

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Precision Melt Analysis Software Only

1845015
2 user licenses, data analysis software for all CFX real-time PCR detection systems, 2 HASP HL keys, installation CD

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Melt Calibration Kit

1845020
Kit includes melt calibration DNA standard, melt primers, instructions

2 1845020 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Amplification-Software/184-5020_0921120543356.xml 1845020 /default/main/bio-rad/WORKAREA/default 09/21/09 12:05 PM en_US jbcbw0n9 2017-11-14 07:07:28 true true internet/product_SKU jbcbw0n9 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Amplification-Software/184-5020_0921120543356.xml LSR 2019-05-05 00:54:20 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555975163991 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:20 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Amplification-Software/184-5020_0921120543356.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Amplification-Software/184-5020_0921120543356.xml 1845020-melt-calibration-kit 1845020 Melt Calibration Kit Melt Calibration Kit Kit includes melt calibration DNA standard, melt primers, instructions Kit includes melt calibration DNA standard, melt primers, instructions Kit includes melt calibration DNA standard, melt primers, instructions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/184-5020_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/184-5020_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/184-5020_feature.jpg ,VN RepEx 184-5020 Kit includes melt calibration DNA standard, melt primers, instructions 1845020, melt, calibration, dna, standard, primers, qpcr, real-time, real, time, pcr, detection, quantification, quantitation, amplification 09/21/09 12:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Amplification-Software N 0 false $599.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845020&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845020&CartQuote=QT&quantity= 1541 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845020&AddToDefaultWishList=true&quantity= 12161500 599.00 1845020
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Precision Melt Analysis™ v1.3 Software Upgrade

SOFT-PM-13-PATCH
Upgrade Precision Melt Analysis Software from version 1.0, 1.1, or 1.2 to version 1.3 for use with CFX Manager™ Software 2.0 and above, or CFX Maestro™ Software 1.0 and above

Number Description Options
6009 High Resolution Melt Parameter Considerations for Optimal Data Resolution, Rev A Click to download
6004 A Practical Guide to High Resolution Melt Analysis Genotyping, Rev A Click to download
10000080911 Instruction Manual, Precision Melt Analysis Software Click to download
5798 Precision Melt Analysis Software Flier, Rev B Click to download
10014810 Instruction Manual, Precision Melt Analysis Software Quick Guide, Rev C Click to download
10015798 Instruction Manual, Melt Calibration Kit, Rev D Click to download
5279 Real-Time PCR Applications Guide, Rev B Click to download

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