Nuvia cPrime Media

Image
nuvia-cprime-media-detail.jpg

Overview

Nuvia cPrime is a mixed-mode ion exchange media designed for versatile capture and high recovery across a wide range of salt concentrations and pH in process-scale applications for biosimilar and biotherapeutic purification.
Read full description Ordering Information

Description

Description

Request Samples

Nuvia cPrime hydrophobic cation exchange media are a new addition to Bio-Rad's family of mixed-mode purification products. These media are designed for process-scale purification of a wide variety of therapeutic proteins. Nuvia cPrime media's unique selectivity allows method developers to use hydrophobic and cation exchange interaction modes to achieve effective purification. More importantly, the media have a large design space for binding and elution, allowing for the development of highly robust methods in a commercial manufacturing setting. Nuvia cPrime media are built on a rigid, mechanically and chemically stable macroporous base matrix with a particle size optimized to deliver exceptional flow properties, fast mass transfer, and stability.

Nuvia cPrime delivers value by providing:

  • New and unique selectivity
  • Salt tolerant media
  • Simple method development
  • Large design space for capture and elution of a variety of biotherapeutic proteins
  • High recovery of target protein
  • Mechanical and chemical stability

Specifications

Specifications

Functional group
Hydrophobic weak cation exchange
Base matrix composition
Macroporous highly crosslinked polymer
Particle size
70 µm ± 10 µm
Dynamic binding capacity
hlgG: >40 mg/ml (at 10% breakthrough, 300 cm/hr)
Lactoferrin: >60 mg/ml
Dynamic binding capacity (lactoferrin)
>60 mg/ml
Ligand density
103–143 µeq/ml
Recommended linear flow rate range
50–600 cm/hr
Pressure vs. flow performance
Under 2 bar at a flow rate of 600 cm/hr
(20 cm × 20 cm packed bed; 1.17 comp. factor)
pH stability short-term
2–14
pH stability long-term
3–13
Chemical stability
1.0 N NaOH, 1.0 N HCl, 25% HOAc, 8 M urea, 6 M GuHCl, 6 M KSCN, 3 M NaCl, 1% Triton X-100, 2% SDS + 0.25 M NaCl, 20% EtOH, 70% EtOH, 30% IPA
Shipping solution
20% ethanol with 30 mM sodium sulfite
Storage conditions
20% ethanol
Shelf life in 20% ethanol
5 years at room temperature

Ordering

Ordering

items

Use the filters below to refine results!

EconoFit_Columns-1x1ml-Sku_TN.png
12009281

Pkg of 1, 1 ml, prepacked hydrophobic cation exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

156-3401_tn.jpg
1563401
25 ml, hydrophobic cation exchange chromatography media, 70 µm particle size
156-3402_tn.jpg
1563402
100 ml, hydrophobic cation exchange chromatography media, 70 µm particle size
156-3404_tn.jpg
1563403
500 ml, hydrophobic cation exchange chromatography media, 70 µm particle size
156-3404_tn.jpg
156-3404
1 L, hydrophobic cation exchange chromatography media, 70 µm particle size
156-3405_tn.jpg
156-3405
5 L, hydrophobic cation exchange chromatography media, 70 µm particle size
156-3406_tn.jpg
156-3406
10 L, hydrophobic cation exchange chromatography media, 70 µm particle size
foresight-unosphere-plate-family-thumb.jpg
7324705
2 x 96-well plates, 20 μl, filter plate prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
foresight-robocolumn-unosphere-200ul-thumb.jpg
7324807
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
foresight-robocolumn-unosphere-600ul-thumb.jpg
7324808
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
foresight-nuvia-cprime-column-732-4722-thumb.jpg
7324722
Pkg of 1, 1 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
foresight-nuvia-cprime-5ml-732-4742-thumb.jpg
7324742
Pkg of 1, 5 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

Documents

Documents

Number Description Options
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
10023853
Instruction Manual, Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin, Rev B
6982
Mixed-Mode Resins/Media Selection Guide
[ Add to Cart (Free) ]
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6242
Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin Product Information Sheet, Rev C
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
6792
Aggregate Purification Resin Selection Card, Ver A
6241
A Purification Strategy for Clinical-Grade Monoclonal Antibody Using Hydrophobic Cation Exchange Chromatography, Rev A
[ Add to Cart (Free) ]
6418
Purification of Recombinant Proteins on Nuvia cPrime Hydrophobic Cation Exchange Resin: A Simple Approach to Method Development, Rev A
[ Add to Cart (Free) ]
6719
Development of an Efficient Manufacturing Process for Adenovirus, Ver A
6720
Development of a cGMP-Ready Purification Process for Adenovirus Purification (Poster), Rev A
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
6807
Practical Guide: Selecting the Optimal Resins for Adenovirus Process Purification, Ver A
6808
Practical Guide: Selecting the Optimal Resins for Aggregate Removal, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6881
Practical Guide: Selecting the Optimal Resins for Removal of DNA Contamination during Process Purification, Ver A
6889
Novel Selectivities and Workflows to Streamline mAb Purification Processes, Ver A
6869
Nuvia™ Chromatography Resins Publications List, Ver A
6946
Monoclonal Antibody Purification: Polish Purification Resins Brochure, Ver A
6887
Quick Monoclonal Antibody Purification Strategy: Non-Affinity Based Capture Flier, Ver A
6880
Quick Virus Purification Strategy: Adenovirus Purification Flier, Ver A