NGC 100 Medium-Pressure Chromatography Systems

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NGC™ 100 ml Chromatography System | Life Science Research

Overview

NGC™ Configurator



Launch the Configurator




BUILD YOUR OWN NGC

The NGC chromatography system introduces a new level of modularity and usability for protein purification.

Use our interactive NGC configurator to design your ideal chromatography system.

Fluidic scheme

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NGC™ System Tour

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NGC Testimonials
NGC™ 100 System Comparison Chart
  NGC Chromatography Systems
Product Description NGC Quest™ 100
(788-0002)
NGC™ Quest 100 Plus
(788-0004)
NGC Scout™ 100
(788-0006)
NGC™ Scout 100 Plus
(788-0008)
NGC Discover™ 100
(788-0010)
NGC™ Discover 100 Pro
(788-0012)
NGC F100 Pump Module
NGC Mixer Module
NGC Sample Inject Valve Module
NGC
Single-Wavelength Detector Module
NGC Buffer Blending Valve Module
NGC pH Valve Module
NGC Sample Pump Module
NGC Inlet Valve Module
NGC Column Switching Valve Module, 100 ml
NGC Outlet Valve Module
NGC™ Systems and Modules — Specifications
System Specifications

Control system ChromLab™ Software
(compatible across all NGC Systems)
Dimensions (D x W x H) 61 x 49 x 56 cm
(NGC Quest™ and NGC Scout™ Systems)
61 x 49 x 74 cm
(NGC Discover™ System)
Weight
(excluding computer)
41–43 kg
(NGC Quest and NGC Scout Systems)
64 kg
(NGC Discover System)
Power supply 100–240 V, 50–60 Hz
Power consumption 750 W maximum

System Pump

Pump type Reciprocating piston
Flow rate setting 10 ml/min pumps: 0.001–10 ml/min (normal range)
100 ml/min pumps: 0.01–100 ml/min (normal range)
Flow rate accuracy ±2% (conditions: 0.1–10 ml/min, pressure
<600 psi (4.1 MPa, 41 bar), viscosity:
0.5–3.7 cP
Pressure range 10 ml/min pumps: 0–25.2 MPa (3,650 psi)
100 ml/min pumps: 0–10 MPa (1,450 psi)
Viscosity range 0.5–10.8 cP (for 10 ml/min and 100 ml/min pumps)

Sample Pump

Pump type Piston pump, metering type
Flow rate setting 0.01–100 ml/min
Flow rate accuracy ±2%
Pressure range 0–10 MPa (1,450 psi)
Viscosity range 0.5–10 cP

Mixer

Mixing principle Chamber with magnetic stirrer
Mixer volume 263 µl (included), 750 µl (included), 2 ml, 5 ml (for F10 pumps)
750 µl (included), 2 ml (included), 5 ml, 12 ml (for F100 pumps)
Gradient composition accuracy ±0.5% (conditions: 3–97% B, 0.25–10 ml/min for F10 pumps)
±0.5% (conditions: 3–97% B, 0.25–100 ml/min for F100 pumps)

Valves

Type Rotary valves and rocker solenoid
Number of valves Up to 12
Functions Loop selection
(PEEK Loop and DynaLoop™ Sample Loop offerings)

Pressure Sensors

Placement of sensors Standard: after system pump
Options: pre-column, post-column
Range 0–3,650 psi
Accuracy ±2 psi or 2%, whichever is greater

Inlet Valves

Inlet A 8 inlets
Inlet B 8 inlets
Sample inlet* Up to 16 inlets


* Coming soon.

UV and UV/Vis Detectors

(each includes an integrated conductivity monitor)
Wavelength Single-wavelength: 255 nm (nucleic acids)
or 280 nm (proteins)
Multi-wavelength (up to 4): 190–800 nm
Absorbance range 0 to >2.8 Au
Linearity 0–2 Au within ±5%
Operating pressure 1,450 psi (10 MPa) for 5, 10 mm flow cells
700 psi (5 MPa) for 2 mm flow cells
Flow cells
Operational interchangeable flow cells available in path lengths of 2, 5, and 10 mm
Preparative: 2 mm (cell volume: 20 µl)
Analytical: 5 mm (cell volume: 9 µl)
Analytical: 10 mm (cell volume: 18 µl)

Conductivity Monitor

Conductivity reading range 0.01 µS/cm–999 mS/cm
Accuracy ±2%
Operating pressure 0–5.5 MPa (800 psi)
Flow cell volume 6 µl
Temperature monitor range 4–100°C
Temperature monitor accuracy ±2%

pH Monitor

pH reading range 0–14
Accuracy ±0.1 pH unit within pH 2–12
Operating pressure 0–70 psi with pH probe inline
and 0–500 psi in bypass mode
Flow cell volume 100 µl (210 µl including internal flow paths)

Column Switching

Five-column valve Can connect up to 5 columns with forward and reverse flow and bypass capability

Buffer Blending

 Buffer blending valve
Standard in the NGC Scout and NGC Discover Systems

Air Sensor

Number of sensors Up to 8 total air sensors (1 for end-of-sample detection, remaining are for end-of-buffer detection)
Accuracy ±0.1 pH unit within pH 2–12
Placement of built-in sensors End of buffer, end of sample
Sensing principle Acoustic


Note:
All NGC Systems include a touch screen and are compatible with the BioFrac™ Fraction Collector and C-96 Autosampler.

Optimized for purifications at the mg to g scale, these preconfigured 100 ml NGC Chromatography Systems are designed to meet a range of throughput and application needs. For scale-up analytical and preparative chromatography.
Read full description

Ordering Information


  • Description
  • Ordering
  • Accessories
  • Fittings
  • Modules
  • Documents

Description

Contact an NGC Specialist
Try an NGC System in your lab

Try an NGC System in your lab

Request a Demo

The family of NGC Medium-Pressure Chromatography Systems fit your throughput requirements when purifying biomolecules for analytical- and preparative-scale applications. Each system is composed of interchangeable modules that confer different system capabilities.

The six preconfigured NGC Liquid Chromatography Systems listed below are designed with increasing automation and throughput to serve a wide range of laboratory needs:

  • NGC Quest 100 (7880002) and NGC Quest 100 Plus Systems (7880004) — for separating biomolecules at high resolution using accurate gradients
  • NGC Scout 100 (7880006) and NGC Scout 100 Plus Systems (7880008) — for medium throughput applications requiring rapid scouting of protein purification conditions with automated gradients and buffer preparation
  • NGC Discover 100 (7880010) and NGC Discover 100 Pro Systems (7880012) — for higher throughput applications with rapid, robust automation and a need for method development

For an overview of the features and capabilities of the six NGC Chromatography Systems, view the NGC 100 System comparison chart and watch the NGC System Tour.

The NGC Systems are composed of interchangeable modules that confer different system capabilities. Browse the module selection guide to help you decide which system is best for you, or use our online tool to configure your personalized NGC Instrument.

Features and Benefits of the NGC 100 ml Chromatography Systems

Flexible, customizable chromatography systems to suit both your application and workflow needs:

  • The guided fluidics selection tool (patent pending), coupled with the module discovery system, enables a single-click application-based system setup through automated module function recognition
  • The Tier Rotate Feature allows optimal placement of valves and detectors to minimize the flow path
  • Compact footprint — fits in a refrigerator, lab bench, or coldroom
  • Point-to-Plumb Feature shows the step-by-step plumbing of the system using LEDs
  • ChromLab Software provides powerful system control, automated methods, and analysis options

Applications and Uses of Medium-Pressure Chromatography Systems

Other NGC Chromatography Systems

Bio-Rad also offers NGC Chromatography Systems with integrated 10 ml system pumps and NGC Fraction Collector as well as a variety of chromatography columns and resins.

Compatible Chromatography Columns

Designed specifically for NGC Chromatography Systems, these prepacked high-resolution columns work at high pressures and are optimized for the separation of biomolecules at fast flow rates.


More Information

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and application requirements:

See how the NGC System benefits University of California researchers by increasing throughput and making it easier for students to become experts at protein purification.


Ordering

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NGC Quest 100 Chromatography System

7880002
Includes 100 ml/min pumps, single-wavelength (UV) and conductivity detection, and automated sample injection, for the purification of proteins

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NGC Quest 100 Plus Chromatography System

7880004
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins

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NGC Scout™ 100 Chromatography System

7880006
Includes 100 ml/min pumps, single-wavelength (UV) and conductivity detection, buffer blending options, for rapid pH scouting during sample purification

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NGC Scout 100 Plus Chromatography System

7880008
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, buffer blending options, and a pH valve, for rapid pH scouting during the purification of proteins, peptides, and nucleic acids

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NGC Discover 100 Chromatography System

7880010
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development

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NGC Discover™ 100 Pro Chromatography System

7880012
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve and column switching of up to 5 columns, for method development

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Accessories

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NGC™ Fraction Collector with Racks

17002070
Pkg of 1, NGC Fraction Collector, includes two 13 mm racks

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NGC Fraction Collector Enclosure

12009848
Protects fractions from dust and particulates.

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$637.00 
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BioFrac™ Fraction Collector

7410002
100/240 V, fraction collector, includes power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac Diverter Valve, fittings kit

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$6,280.00 
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Computer

7884015
PC for ChromLab™ Software and database, communicates with and stores data generated by the NGC™ System

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$2,262.00 
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Monitor, 22"

7884027
22" monitor for use with 788-4015, for viewing chromatograms and actions performed on the PC

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$439.00 
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NGC™ Communication Adaptor

7884025
Pkg of 1, enables communication between the NGC™ Systems and accessories like the BioFrac™ Fraction Collector

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$395.00 
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NGC™ Touch Screen Stand

7885040
Pkg of 1, for mounting touch screen to NGC Systems

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$647.00 
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NGC Column Holder

7885038
Pkg of 1, magnetic holder, holds long columns and mounts on the front or the side of instrument

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$175.00 
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NGC Dual Column Holder

7885057
Pkg of 1, magnetic holder, dual clamp for holding long columns and mounts on the front or the side of instrument

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$676.00 
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NGC™ Tubing Organizer, Large

7885035
Pkg of 3, guides and holds 1/8" inlet tubing in an organized manner, for use with NGC Chromatography Systems

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$185.00 
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NGC™ Tubing Organizer, Small

7885042
Pkg of 3, guides and holds 1/16" inlet tubing in an organized manner, for use with NGC Chromatography Systems

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$185.00 
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NGC™ Cartridge Holder

7885039
Pkg of 1, flexible magnetic holder, holds 1–10 ml cartridges and mounts on the front or the side of instrument

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$78.00 
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NGC™ Sample/Wash Tube Holder

7885041
Pkg of 1, holds two 50 ml Falcon Tubes, for use with NGC Systems

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$78.00 
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Bottle Cap Kit

7500566
Fittings kit, includes 2 bottle caps, 2 plugs, for use with BioLogic DuoFlow™ and NGC™ Systems

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$103.00 
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Inline Filter Kit

7500703
Filter kit, includes 1 inline filter and 2 replacement frits, for use with BioLogic DuoFlow™ and NGC™ Systems

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$108.00 
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Replacement Frits

7500704
Pkg of 5, replacement fittings, for inline filter kit

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$34.00 
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NGC™ Signal Import Module

7884016
Pkg of 1, enables analog to digital signal conversion and connection to external detectors and third party autosamplers such as the C-96 Autosampler

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$1,500.00 
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Autosampler Cable for NGC™ Signal Import Module

7885013
Pkg of 1, autosampler cable for NGC Signal Import Module

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$146.00 
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40 psi Backpressure Regulator

7500230
Pkg of 1, for use with BioLogic DuoFlow™ and NGC™ Workstations

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$185.00 
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NGC™ pH Probe (Ag/AgCl)

7885026
Pkg of 1, for accurate pH monitoring (pH 1–14) during purifications performed to 200 ml/min, for use with the pH valve

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$500.00 
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NGC™ Buffer Tray

7885014
Pkg of 1, holds up to 8 buffer bottles, includes drain port to prevent flooding in the event of a leak

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$185.00 
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NGC™ Drip Tray

7885016
Pkg of 1, for use with NGC Systems

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$185.00 
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NGC™ 3rd Tier Expansion Frame

7884000
Pkg of 1, expands a 2-tier NGC System to a 3-tier system for adding extra plug-and-play modules

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$2,898.00 
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NGC™ 4th Tier Expansion Frame

7884001
Pkg of 1, expands a 3-tier NGC System to a 4-tier system for adding extra plug-and-play modules

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$2,898.00 
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NGC™ Blank Module

7884005
Pkg of 1, blank module, for covering unused bays on the base and expansion layers

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$236.00 
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NGC UV and Conductivity Flow Cell, 10 mm

12012531
Pkg of 1, includes a 10 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for analytical applications.

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$1,200.00 
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NGC UV and Conductivity Flow Cell, 5 mm

12012532
Pkg of 1, includes a 5 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module. (12010343)

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$1,200.00 
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NGC UV and Conductivity Flow Cell, 2 mm

12012533
Pkg of 1, includes a 2 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for preparative applications.

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$1,800.00 
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Fittings

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list price. If the problem persists, please call customer service at or send an
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NGC™ Fittings Kit

7884017
Pkg of 1, kit includes multiple fittings, fittings maker, fittings tightener, ferrules, nuts, screwdrivers, pump head keys, and syringes required to set up, prepare, and prime NGC Systems

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$950.00 
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Luer to BioLogic™ and NGC™ Systems Fittings Kit

7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems

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$68.00 
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Fittings Maker, Magnetic

7885043
For making fittings for 1/16" (1.6 mm) OD and 1/8" (3.2 mm) tubing

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$214.00 
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Fittings Tightener, Short

7885008
Pkg of 1, short tool for tightening hard-to-reach fittings

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$5.00 
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BioLogic DuoFlow™ and NGC™ Fittings Tool

7500560
Pkg of 1, fittings tool, for use with BioLogic DuoFlow and NGC Systems

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$14.00 
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PEEK Nut 1/16"

7885025
Pkg of 20, part of NGC™ fittings kit, for use with NGC Chromatography Systems

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$4.00 
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Ferrule and Lock Ring

7500556
Pkg of 10, ferrule and lock ring, for 1/16" OD (1.6 mm) tubing, for use with BioLogic DuoFlow™ and NGC™ Systems

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$77.00 
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PEEK Nut 1/8"

7885015
Pkg of 10, part of NGC™ Fittings Kit, for use with NGC Chromatography Systems

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$4.00 
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PEEK Ferrule 1/8"

7885055
Pkg of 15, part of NGC™ fittings kit, for use with NGC Chromatography Systems

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$4.00 
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Tefzel Plug

7500563
Pkg of 5, 1/4–28 male connection, to plug unused ports on valves and columns, for use with BioLogic DuoFlow™ and NGC™ Systems

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$27.00 
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10-32 PEEK Union 1/16 in

7885007
Pkg of 2, union for NGC™ Systems, 0.020" ID

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$67.00 
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1/4-28 F to 10-32 M Adaptor and 1/4-28 F to 10-32 F Adaptor

7885009
Pkg of 4 (qty 2 pairs — each with one 1/4-28 F to 10-32 M adaptor and one 1/4-28 M to 10-32 F adaptor)

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$69.00 
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Fitting Adapters ¼"-28F to 10-32M

7500564
Pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic DuoFlow

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$69.00 
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Econo-Column® to BioLogic DuoFlow™ and NGC™ Systems Fittings Kit

7500565
Pkg of 2, fittings to connect one Econo-Column (luer) Column to BioLogic DuoFlow and NGC Systems

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$69.00 
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Adaptor, FEM Slip Luer-FEM 10-32 and Adaptor, Quick Connect M, Luer-FEM 10-32

7885010
Pkg of 10, includes five slip luer F to 10-32 M adaptors and five quick-connect luer M to 10-32 F adaptors

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$203.00 
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Long Fingertight Fittings

7601308
Fitting for tubing

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$46.00 
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Long Fingertight Fittings

7601311
Fitting for tubing

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$60.00 
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Tefzel Cap

7500559
Pkg of 5, Tefzel caps, 1/4–28 female connection, to plug unused tubing, for use with BioLogic DuoFlow™ and NGC™ Systems

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$40.00 
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Tefzel Union Adaptor

7500561
Pkg of 2, 1/4–28 to M6, to connect one M6 column to BioLogic DuoFlow™ and NGC™ Systems

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$30.00 
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Tefzel Union

7500562
Pkg of 1, 1/4–28 to 1/4–28 to extend tubing, for use with BioLogic DuoFlow™ and NGC™ Systems

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$60.00 
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UNO® 10-32 Fittings Kit

7500568
Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow™ and NGC™ Systems

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$54.00 
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NGC Multi-Wavelength Detector Module

12010343
Pkg of 1, includes UV/Vis and conductivity detector kit with necessary tubing and fittings, for protein, peptide, nucleic acid, and chromophore detection

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NGC Single-Wavelength Detector Module

7884008
Pkg of 1, includes UV/conductivity detector kit with necessary tubing and fittings, for nucleotide and protein detection, and salt gradient monitoring

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$5,740.00 
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NGC™ pH Valve Module

7884011
Pkg of 1, contains the pH valve kit, includes pH probe, tubing, fittings, for accurate inline pH measurement

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$4,756.00 
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NGC™ Sample Inject Valve Module

7884007
Pkg of 1, kit includes necessary tubing, fittings, and a sample injection port, for automated application of small sample volume samples

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$3,016.00 
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