NGC 100 Medium-Pressure Chromatography Systems
Optimized for purifications at the mg to g scale, these preconfigured 100 ml NGC Chromatography Systems are designed to meet a range of throughput and application needs.
Overview
The family of NGC Medium-Pressure Chromatography Systems fit your throughput requirements when purifying biomolecules at laboratory-scale. Each system is composed of interchangeable valves and pumps that confer different system capabilities.
The preconfigured NGC Liquid Chromatography Systems listed below are designed with increasing automation and throughput to serve a wide range of laboratory needs:
- NGC Quest 100 Plus Systems (7880004) — for separating biomolecules at high resolution using accurate gradients
- NGC Scout 100 Plus Systems (7880008) — for medium throughput applications requiring rapid scouting of protein purification conditions with automated gradients and buffer preparation
- NGC Discover 100 (7880010) and NGC Discover 100 Pro Systems (7880012) — for higher throughput applications with rapid, robust automation and a need for method development
For an overview of the features and capabilities of the four NGC Chromatography Systems, view the NGC 100 System comparison chart and watch the NGC System Tour.
The NGC Systems are composed of interchangeable modules that confer different system capabilities. Browse the module selection guide to help you decide which system is best for you, or use our online tool to configure your personalized NGC Instrument.
Features and Benefits of the NGC 100 ml Chromatography Systems
Flexible, customizable chromatography systems to suit both your application and workflow needs:
- The guided fluidics selection tool (patent pending), coupled with the module discovery system, enables a single-click application-based system setup through automated module function recognition
- The Tier Rotate Feature allows optimal placement of valves and detectors to minimize the flow path
- Compact footprint — fits in a refrigerator, lab bench, or coldroom
- Point-to-Plumb Feature shows the step-by-step plumbing of the system using LEDs
- ChromLab Software provides powerful system control, automated methods, and analysis options
Applications and Uses of Medium-Pressure Chromatography Systems
- Preparative chromatography
- Protein purification and isolation
Other NGC Chromatography Systems
Bio-Rad also offers NGC Chromatography Systems with integrated 10 ml system pumps and NGC Fraction Collector as well as a variety of chromatography columns and resins.
Compatible Chromatography Columns
Designed specifically for NGC Chromatography Systems, these prepacked high-resolution columns work at high pressures and are optimized for the separation of biomolecules at fast flow rates.
More Information
Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and application requirements:
- Higher flow rates
- More sophisticated detection capabilities
- pH monitoring
- Column scouting
- Buffer blending
See how the NGC System benefits University of California researchers by increasing throughput and making it easier for students to become experts at protein purification.
Ordering
items
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Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, buffer blending options, and a pH valve, for rapid pH scouting during the purification of proteins, peptides, and nucleic acids
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve and column switching of up to 5 columns, for method development
Accessories
Pkg of 1, NGC Fraction Collector, includes two 13 mm racks
100/240 V, fraction collector, includes power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac Diverter Valve, fittings kit
PC for ChromLab Software and database, communicates with and stores data generated by the NGC System
Pkg of 1, enables communication between the NGC Systems and accessories like the BioFrac Fraction Collector
Pkg of 1, for mounting touch screen to NGC Systems
Pkg of 3, guides and holds 1/8" inlet tubing in an organized manner, for use with NGC Chromatography Systems
Pkg of 3, guides and holds 1/16" inlet tubing in an organized manner, for use with NGC Chromatography Systems
Pkg of 1, flexible magnetic holder, holds 1–10 ml cartridges and mounts on the front or the side of instrument
Pkg of 1, holds two 50 ml Falcon Tubes, for use with NGC Systems
Fittings kit, includes 2 bottle caps, 2 plugs, for use with BioLogic DuoFlow and NGC Systems
Filter kit, includes 1 inline filter and 2 replacement frits, for use with BioLogic DuoFlow and NGC Systems
Pkg of 1, enables analog to digital signal conversion and connection to external detectors and third party autosamplers such as the C-96 Autosampler
Pkg of 1, for use with BioLogic DuoFlow and NGC Workstations
Pkg of 1, for accurate pH monitoring (pH 1–14) during purifications performed to 200 ml/min, for use with the pH valve
Pkg of 1, holds up to 8 buffer bottles, includes drain port to prevent flooding in the event of a leak
Pkg of 1, for use with NGC Systems
Pkg of 1, expands a 2-tier NGC System to a 3-tier system for adding extra plug-and-play modules
Pkg of 1, expands a 3-tier NGC System to a 4-tier system for adding extra plug-and-play modules
Pkg of 1, blank module, for covering unused bays on the base and expansion layers
Pkg of 1, includes a 10 mm UV/Vis flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343). The 10 mm flow cell is necessary for analytical applications on the NGC.
Pkg of 1, includes a 5 mm UV/Vis flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module for standard preparative applications (12010343)
Pkg of 1, includes a 2 mm UV/Vis flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for scale-up of preparative applications
Pkg of 1, UV lamp replacement for multi-wavelength detectors
Pkg of 1, visible lamp replacement for multi-wavelength detectors
Pkg of 1, 255/280 nm LED lamp replacement for single-wavelength UV detectors
Pkg of 1, 22-gauge, injection needle, blunt, for use with the NGC Chromatography System
Pkg of 1, sample injection port, for use with NGC Chromatography System
Fittings
Pkg of 1, kit includes multiple fittings, fittings maker, fittings tightener, ferrules, nuts, screwdrivers, pump head keys, and syringes required to set up, prepare, and prime NGC Systems
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and NGC Systems
Pkg of 1, fittings tool, for use with BioLogic DuoFlow and NGC Systems
Pkg of 20, part of NGC fittings kit, for use with NGC Chromatography Systems
Pkg of 10, ferrule and lock ring, for 1/16" OD (1.6 mm) tubing, for use with BioLogic DuoFlow and NGC Systems
Pkg of 10, part of NGC Fittings Kit, for use with NGC Chromatography Systems
Pkg of 15, part of NGC fittings kit, for use with NGC Chromatography Systems
Pkg of 5, 1/4–28 male connection, to plug unused ports on valves and columns, for use with BioLogic DuoFlow and NGC Systems
Pkg of 2, union for NGC Systems, 0.020" ID kg of 2, union for NGC Systems
Pkg of 2, fittings to connect one Econo-Column (luer) Column to BioLogic DuoFlow and NGC Systems
Pkg of 5, Tefzel caps, 1/4–28 female connection, to plug unused tubing, for use with BioLogic DuoFlow and NGC Systems
Pkg of 2, 1/4–28 to M6, to connect one M6 column to BioLogic DuoFlow and NGC Systems
Pkg of 1, 1/4–28 to 1/4–28 to extend tubing, for use with BioLogic DuoFlow and NGC Systems
Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow and NGC Systems
Modules
Pkg of 1, includes UV/Vis and conductivity detector kit with 5 mm flow cell, necessary tubing, and fittings, for protein, peptide, nucleic acid, and chromophore detection
Pkg of 1, contains the pH valve kit, includes pH probe, tubing, fittings, for accurate inline pH measurement
Pkg of 1, kit includes necessary tubing, fittings, and a sample injection port, for automated application of small sample volume samples
Pkg of 1, kit includes necessary tubing and fittings, for automated switching between multiple buffers and samples during method development
Pkg of 1, kit includes necessary tubing and fittings for automated fraction collection of large-volume fractions with up to 12 vessels
Pkg of 1, kit includes the necessary tubing and fittings to accommodate up to 5 columns, for column scouting and reverse flow applications using NGC 100 Chromatography Systems
Pkg of 1, blank module, for covering unused bays on the base and expansion layers
Pkg of 1, kit includes two large air sensors, protects against air entering pumps and columns
Pkg of 1, part of the NGC Air Sensor Module, protects against air entering pumps and columns
Pkg of 1, includes 100 ml/min system pump kit with the necessary tubing and fittings, for creating buffer gradients
Pkg of 1, includes 100 ml/min sample pump kit with the necessary tubing and fittings, for automated large-volume sample application
Pkg of 1, compatible with F10 and F100 NGC Chromatography Systems.