Model 491 Prep Cell and Mini Prep Cell

Image
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Overview

Fractionation by size (molecular weight) is an effective enrichment strategy for studies of protein families and posttranslational modifications, because the sizes of these proteins tend to be similar.
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Description

Description

Fractionation by size (molecular weight) is an effective enrichment strategy for studies of protein families and posttranslational modifications, because the sizes of these proteins tend to be similar.

The Model 491 Prep Cell and Mini Prep Cell separate biomolecules quickly and efficiently by continuous-elution electrophoresis. Samples are electrophoresed through a cylindrical gel matrix, where they are separated into ring-shaped bands. As individual bands migrate off the bottom of the gel, they are collected in discrete liquid fractions available for assay and characterization.

The Model 491 Prep Cell and Mini Prep Cell:

  • Allow resolution of proteins differing in molecular weight by as little as 2%
  • Separate up to 500 mg of total protein
  • Can be used as a complementary separation strategy to 2-D gels as well as for downstream protein purification

The Model 491 Prep Cel has a sample capacity of 1–500 mg/0.5–15 ml.

The Mini Prep Cell has a sample capacity of 0.5–1,000 µg/50–500 µl.

Accessory equipment required to run the Model 491 and Mini Prep Cells includes a fraction collector and power supply such as the PowerPac HV power supply. The Model 491 Prep Celll also requires a peristaltic pump.

Ordering

Ordering

items

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(Discontinued) Model 491 Prep Cell
1702926
100/120 V, continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume) includes pump, starter kit with protein standard
170-2927_tn.jpg
(Discontinued) Model 491 Prep Cell
1702927
220/240 V, continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume) includes pump, starter kit with protein standard

Note: power cord not included in U.S.; 220 V cord with appropriate plug is provided only for international customers
170-2928_tn.jpg
1702928
Continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume), includes gel casting equipment
170-2915_tn.jpg
1702915
Continuous-elution electrophoresis cell, 0.5-1.0 mg/50–500 µl sample capacity (mass/volume), includes reagent starter kit
170-2908_tn.jpg
1702908
Continuous-elution electrophoresis cell, 0.5-1.0 mg/50–500 µl sample capacity (mass/volume), includes gel casting equipment

Accessories

Accessories

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1702912
Pkg of 1 assembly, includes elution collection tubing, for use with mini prep cell (#170-2915, 170-2908)
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1702939
Kit includes sample loading guide and syringe with PTFE tubing, for use with Model 491 prep cell (#170-2926, 170-2927)
170-2935_tn.jpg
1702935
Kit includes stopcock with tubing, 3 elution buffer circulation lines, electrophoresis/cooling buffer circulation lines, connectors
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1610323
1 ml, prestained mixture of trypsin inhibitor and carbonic anhydrase for use with the prep cell starter kit (#161-5101)
1615101
SDS-PAGE reagents for Model 491 prep cell, includes running buffer, Tris, APS, TEMED, bis-acrylamide, acrylamide

Kits & Reagents

Kits & Reagents

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1610122
30 g, 37.5:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder

Documents

Documents

Number Description Options
M1702925
Instruction Manual, Model 491 Prep Cell, Rev D
4006032
Instruction Manual, Mini Prep Cell
LIT466
Instruction Manual, Mini Prep Cell and Model 491 Prep Cell Starter Kit
10044265
Mini Prep Cell Assembly Guide, Rev B
1964
Model 491 Prep Cell and Mini Prep Cell Brochure, Rev C
3161
Model 491 Prep Cell Bio-Rad Literature